Kangiella shandongensis sp. nov., a novel species isolated from saltern in Yantai, China

• Published in: Antonie van Leeuwenhoek 2022, Vol.115 (1), p.33-40
• Main Authors: Peng, Li-Yang, Yin, Rui, Gao, Shu-Kun, Jiang, Hui-Ning, Liu, Xiao-Xiao, Ma, Yu, Zhou, Yan-Xia
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Cardiolipin; DNA, Bacterial - genetics; Fatty acids; Gene sequencing; Genomic analysis; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nucleotide sequence; Nucleotides; Original Paper; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogeny; Plant Sciences; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Salterns; Sequence Analysis, DNA; Sodium chloride; Soil Science & Conservation
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01682-9

A Gram-stain-negative, wheat, rod-shaped, non-motile, non-spore forming, and facultatively anaerobic bacterium strain, designated as PI T , was isolated from saline silt samples collected in saltern in Yantai, Shandong, China. Growth was observed within the ranges 4–45 °C (optimally at 33 °C), pH 6.0–9.0 (optimally at pH 7.0) and 1.0–11.0% NaCl (optimally at 3.0%, w/v). Strain PI T showed highest 16S rRNA gene sequence similarity to Kangiella sediminilitoris BB-Mw22 T (98.3%) and Kangiella taiwanensis KT1 T (98.3%). The major cellular fatty acids (> 10% of the total fatty acids) were iso-C 15:0 (52.7%) and summed featured 9 (iso-C 17:1 ω 9 c /C 16:0 10-methyl, 11.8%). The major polar lipids identified were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol. The major respiratory isoprenoid quinone was Q-8. The G + C content of the genomic DNA was 45.8%. Average Nucleotide Identity values between whole genome sequences of strain PI T and next related type strains supported the novel species status. Based on physiological, biochemical, chemotaxonomic characteristics and genomic analysis, strain PI T is considered to represent a novel species within the genus Kangiella , for which the name Kangiella shandongensis sp. nov. is proposed. The type strain is PI T (= KCTC 82509  T  = MCCC 1K04352 T ).