CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

[Display omitted] •CRISPR/Cas9 research in Bombyx mori, as model organism, leads the development of Lepidoptera.•CRISPR/Cas9 provides opportunities for applications in lepidopteran insects.•CRISPR/Cas9 has limitations in the application of Lepidoptera. Clustered regularly spaced short palindrome rep...

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Bibliographic Details
Published in:Journal of insect physiology 2021-11, Vol.135, p.104325-104325, Article 104325
Main Authors: Li, Jiang-Jie, Shi, Yan, Wu, Ji-Nan, Li, Hao, Smagghe, Guy, Liu, Tong-Xian
Format: Article
Language:English
Subjects:
Animals
Bombyx - genetics
CRISPR-Cas Systems
CRISPR/Cas9 system
Gene Editing
Lepidoptera
Lepidoptera - genetics
Prospects
Research progress
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Summary:[Display omitted] •CRISPR/Cas9 research in Bombyx mori, as model organism, leads the development of Lepidoptera.•CRISPR/Cas9 provides opportunities for applications in lepidopteran insects.•CRISPR/Cas9 has limitations in the application of Lepidoptera. Clustered regularly spaced short palindrome repeats (CRISPR) structure family forms the acquired immune system in bacteria and archaea. Recent advances in CRISPR/Cas genome editing as derived from prokaryotes, confirmed the characteristics of robustness, high target specificity and programmability, and also revolutionized the insect sciences field. The successful application of CRISPR in a wide variety of lepidopteran insects, with a high genetic diversity, provided opportunities to explore gene functions, insect modification and pest control. In this review, we present a detailed overview on the recent progress of CRISPR in lepidopteran insects, and described the basic principles of the system and its application. Major interest is on wing development, pigmentation, mating, reproduction, sex determination, metamorphosis, resistance and silkworm breeding innovation. Finally, we outlined the limitations of CRISPR/Cas system and discussed its application prospects in lepidopteran insects.
ISSN:0022-1910
1879-1611
DOI:10.1016/j.jinsphys.2021.104325