A new biomimetic nanozyme of hemin/graphdiyne oxide with superior peroxidase-like activity for colorimetric bioassays

• Published in: Analyst (London) 2021-11, Vol.146 (23), p.7284-7293
• Main Authors: Zhu, Qiqi, Yuan, Yonghua, Yan, Bin, Zhou, Jing, Zuo, Jianli, Bai, Lijuan
• Format: Article
• Language: English
• Subjects: Allotropy; Bioassays; Biomimetics; Colorimetry; Glucose; Hydrogen peroxide; Nanocomposites; Nanomaterials; Peroxidase
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d1an01456k

Graphdiyne oxide (GDYO) is a novel type of two-dimensional carbon allotrope nanomaterial consisting of a large conjugated system and excellent chemical stability. To date, application of GDYO as a nanozyme in biosensing has been rarely reported. In this study, a novel ultrasensitive colorimetric bioassay was constructed using a hemin/GDYO nanocomposite (H/GDYO) as a new nanozyme with superior peroxidase-like activity for the detection of H 2 O 2 and glucose. It was discovered that H/GDYO exhibited 6-fold higher peroxidase-like activity than pure hemin. Catalytic kinetic analysis showed that H/GDYO had a much higher affinity for H 2 O 2 and glucose than that of hemin. The designed colorimetric bioassay displayed excellent sensitivity for H 2 O 2 and glucose detection with a wide linear range of 0.015-0.5 mM and 0.1-10 mM, respectively, while the limit of detection (LOD) was as low as 4.39 μM and 38 μM, respectively. Moreover, it was successfully applied for the analysis of H 2 O 2 in milk and glucose in real human serum samples with acceptable recoveries. Importantly, the developed colorimetric bioassay shows good agreement with the results obtained from a commercial blood glucose meter. We believe that the proposed method could provide a promising prospect for medical diagnosis and biotechnology. This work describes a novel colorimetric biosensor for ultrasensitive detection of H 2 O 2 and glucose using a hemin/GDYO nanocomposite as a new biomimetic nanozyme with superior peroxidase-like activity.