Novel Methods for Detecting Human Cholesterol Crystals from Sampled Blood

Abstract Objective Nonobstructive general angiography (NOGA) is a novel modality to detect and sample spontaneous ruptured aortic plaques (SRAPs). We aimed to establish novel methods to detect cholesterol crystals (CCs) in sampled SRAPs. Methods Blood specimens containing SRAPs were obtained from pa...

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Bibliographic Details
Published in:Laboratory medicine 2022-05, Vol.53 (3), p.255-261
Main Authors: Iwa, Nobuzo, Yutani, Chikao, Komatsu, Sei, Takahashi, Satoru, Takewa, Mitsuhiko, Ohara, Tomoki, Kodama, Kazuhisa
Format: Article
Language:English
Subjects:
Aorta - chemistry
Aorta - pathology
Atherosclerosis
Cholesterol
Humans
Methods
Microscopy
Plaque, Atherosclerotic - diagnosis
Plaque, Atherosclerotic - pathology
Water
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Summary:Abstract Objective Nonobstructive general angiography (NOGA) is a novel modality to detect and sample spontaneous ruptured aortic plaques (SRAPs). We aimed to establish novel methods to detect cholesterol crystals (CCs) in sampled SRAPs. Methods Blood specimens containing SRAPs were obtained from patients using NOGA. Blood was instantly frozen on a glass slide and subsequently thawed for quantitative analysis and spread onto a filter paper that was rinsed using distilled water. Qualitative analysis was performed for the rinsed water using polarized light microscopy, and the filter paper was embedded in paraffin for histologic analysis. Results The CCs were clearly observed after hemolysis using the instant freeze-thaw method. The filter paper rinse method indicated free CCs of varying shapes under polarized light microscopy without erythrocytes. On the filter paper, sampled SRAPs showed Lamé-like small particles. Histopathology revealed various atheromatous components. Conclusion A set of novel methods for detecting CCs from sampled blood was established.
ISSN:0007-5027
1943-7730
DOI:10.1093/labmed/lmab078