Anti‐IgE effect of small‐molecule‐compound arctigenin on food allergy in association with a distinct transcriptome profile

Background Excessive production of IgE plays a major role in the pathology of food allergy. In an attempt to identify anti‐IgE natural products, Arctium Lappa was one of the most effective herbs among approximately 300 screened medicinal herbs. However, little is known about its anti‐IgE compounds....

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Published in:Clinical and experimental allergy 2022-02, Vol.52 (2), p.250-264
Main Authors: Cao, Mingzhuo, Liu, Changda, Srivastava, Kamal. D., Lin, Adora, Lazarski, Christopher, Wang, Lu, Maskey, Anish, Song, Ying, Chen, Xiaoke, Yang, Nan, Zambrano, Linda, Bushko, Renna, Nowak‐Wegrzyn, Anna, Cox, Amanda, Liu, Zhigang, Huang, Weihua, Dunkin, David, Miao, Mingsan, Li, Xiu‐Min
Format: Article
Language:English
Subjects:
Animal models
Animals
Antibodies, Anti-Idiotypic
anti‐inflammatory
Arctigenin
Arctium
B and T cell regulation
Bcl-6 protein
Cell cycle
Cell division
Food allergies
Food Hypersensitivity - drug therapy
Furans
Herbal medicine
Histamine
Humans
Hypothermia
IgE inhibition
Immunoglobulin E
Inflammation
Lignans
Lymphocytes T
Medicinal plants
Mice
Myeloma
Natural products
Patients
peanut allergy
Peanut Hypersensitivity - drug therapy
Plant Extracts - chemistry
Transcriptome
Transcriptomes
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Summary:Background Excessive production of IgE plays a major role in the pathology of food allergy. In an attempt to identify anti‐IgE natural products, Arctium Lappa was one of the most effective herbs among approximately 300 screened medicinal herbs. However, little is known about its anti‐IgE compounds. Objective To identify compounds from Arctium Lappa for targeted therapy on IgE production and explore their underlying mechanisms. Methods Liquid‐liquid extraction and column chromatographic methods were used to purify the compounds. IgE inhibitory effects were determined on IgE‐producing human myeloma U266 cells, peanut‐allergic murine model and PBMCs from food‐allergic patients. Genes involved in IgE inhibition in PBMCs were studied by RNA sequencing. Results The main compounds isolated were identified as arctiin and arctigenin. Both compounds significantly inhibited IgE production in U266 cells, with arctigenin the most potent (IC50=5.09μg/mL). Arctigenin (at a dose of 13 mg/kg) markedly reduced peanut‐specific IgE levels, blocked hypothermia and histamine release in a peanut‐allergic mouse model. Arctigenin also significantly reduced IgE production and Th2 cytokines (IL‐5, IL‐13) by PBMCs. We found 479 differentially expressed genes in PBMCs with arctigenin treatment (p 
ISSN:0954-7894
1365-2222
DOI:10.1111/cea.14048