In cellulo synthesis of dendrimeric sensors for fluorescence-on imaging of bacterial phagocytosis

Methods for optical tracking of pathogen–host interactions are of biomedical significance. We herein have reported a high molecular weight pH sensor (Den-pH) that is assembled in bacteria and then stably trapped in bacteria irrespective of bacterial membrane potentials. Endowed with acidity-triggere...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2022-06, Vol.10 (25), p.4800-4809
Main Authors: Jiang, Feng, Shi, Yilong, Zou, Xiaoxue, Han, Jiahuai, Han, Shoufa
Format: Article
Language:English
Subjects:
Acidity
Bacteria
Fluorescence
Liability
Macrophages
Molecular weight
Optical tracking
Pathogens
pH effects
pH sensors
Phagocytosis
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Summary:Methods for optical tracking of pathogen–host interactions are of biomedical significance. We herein have reported a high molecular weight pH sensor (Den-pH) that is assembled in bacteria and then stably trapped in bacteria irrespective of bacterial membrane potentials. Endowed with acidity-triggered red fluorescence, Den-pH allows signal-on tracking of S. aureus in phagocytosis by macrophages. Intra-bacterial formation of multifunctional optical probes, which offers the advantage of overcoming the liability of conventional potential-sensitive dyes to dissipate from stressed bacteria, offers a new tool to study stressed pathogens.
ISSN:2050-750X
2050-7518
DOI:10.1039/d1tb02012a