The Microbiota of Ixodes ricinus and Dermacentor reticulatus Ticks Collected from a Highly Populated City of Eastern Europe

• Published in: Microbial ecology 2022-11, Vol.84 (4), p.1072-1086
• Main Authors: Elias, Leta, Hearn, Aimee-Joy M., Blazier, John C., Rogovska, Yuliya V., Wang, Jiangli, Li, Sijia, Liu, Shuling, Nebogatkin, Igor V., Rogovskyy, Artem S.
• Format: Article
• Language: English
• Subjects: Animal species; Anthropogenic factors; Biomedical and Life Sciences; Dermacentor reticulatus; Ecology; Environmental Microbiology; Geoecology/Natural Processes; Ixodes ricinus; Land management; Life Sciences; Microbial Ecology; Microbiology; Microbiota; Nature Conservation; Regional analysis; Regional planning; Regional variations; Regions; rRNA 16S; Rural areas; Sex; Statistical analysis; Ticks; Translocation; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-021-01921-6

Recent investigations have examined, through sequencing the V6 region of 16S rRNA gene, the microbiota of questing Ixodes ricinus and Dermacentor reticulatus ticks collected from rural areas of Central (Dnipropetrovs’k (region D) and Poltava (region P)) and Northeastern (Kharkiv (region K)) Ukraine. In addition to defining the bacterial microbiota of both tick species, the previous investigations also revealed a high degree of inter-sex and inter-regional variations in the tick microbiota. As a continuation of the two studies, the present investigation has analyzed individual microbiota of questing I. ricinus ( n  = 50) and D. reticulatus ( n  = 50) ticks originating from Kyiv, the largest city of Ukraine. The Kyiv tick microbiota were compared between males and females for each tick species. Additionally, a cross-regional analysis was performed to compare the microbiota of Kyiv ticks to those from regions D, K, and P. Numerous statistically significant inter-sex and inter-regional variations were detected when alpha diversity, beta diversity, the bacterial relative and differential abundances were assessed. The overall results demonstrated that the microbiota of Kyiv ticks were statistically different compared to the ticks of the other three regions. Besides existing climatic and geographical differences between the four regions, the authors hypothesize that various anthropogenic factors of the megapolis (e.g., animal species translocation, land management, ecology) could have contributed to the distinct microbiota of Kyiv ticks observed in this study.