The role of NFKB1/NFKBIA genetic variants in HPV infection: A cross-sectional cohort study

• Published in: Experimental and molecular pathology 2022-02, Vol.124, p.104716-104716, Article 104716
• Main Authors: Sena, Michelle Mota, Trugilo, Kleber Paiva, Okuyama, Nádia Calvo Martins, Pereira, Érica Romão, Cezar-dos-Santos, Fernando, Ferreira, Rodolfo Sanches, Esposito, Aline, Pereira, Ana Paula Lombardi, d'Oliveira Couto-Filho, José, Watanabe, Maria Angelica Ehara, de Oliveira, Karen Brajão
• Format: Article
• Language: English
• Subjects: Adult; Cervical cancer; Cohort Studies; Cross-Sectional Studies; DNA, Viral - analysis; Female; Genetic Association Studies; Genetic polymorphism; Genetic Predisposition to Disease; Humans; Middle Aged; NF-kappa B; NF-kappa B p50 Subunit - genetics; NF-KappaB Inhibitor alpha - genetics; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Papillomaviridae - pathogenicity; Papillomavirus infections; Papillomavirus Infections - diagnosis; Papillomavirus Infections - genetics; Papillomavirus Infections - pathology; Polymorphism, Single Nucleotide; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - etiology; Uterine Cervical Neoplasms - pathology
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2021.104716

Human Papillomavirus (HPV) is the most frequent etiological agent sexually transmitted. In the context of the immune response, NF-kB pathway plays an important role controlling the expression of several genes essential to cellular activity and structural and/or functional changes in components of this pathway can promote the development of several tumors. Thus, the study purpose was to evaluate the influence of NFKB1 rs28362491 and NFKBIA rs696 genetic variants on HPV infection and cervical lesions development. In this study 334 patients were recruited, of whom 48.8% (n = 163) were HPV infected, and considered our case group. HPV-DNA was detected by polymerase chain reaction (PCR) and the genetic variants were assessed in blood cells and tumor tissues paraffin embedded samples through restriction fragment length polymorphism analysis. Among women who were recruited for this study who were infected, 37.4% presented precursor lesions and 16.8% were diagnosed with cervical cancer (CC). The present study did not observe significant effects of the interaction between such genetic variants on HPV infection, nor on the development of lesions and progression to CC. Further studies will be important to investigate if under some circumstance the NFKB1 rs28362491 and NFKBIA rs696 genetic variants influence the progression of HPV-associated lesions.