Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus)

Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS)...

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Published in:Genomics (San Diego, Calif.) Calif.), 2021-11, Vol.113 (6), p.4303-4312
Main Authors: Herkenhoff, Marcos Edgar, Bovolenta, Luiz A., Broedel, Oliver, dos Santos, Lucilene D., de Oliveira, Arthur C., Chuffa, Luiz G.A., Ribeiro, Amanda de O., Lupi, Luiz A., Dias, Marco A.D., Hilsdorf, Alexandre W.S., Frohme, Marcus, Pinhal, Danillo
Format: Article
Language:English
Subjects:
Animals
Cichlids - genetics
Cichlids - metabolism
ESI-Q-TOF
Growth
Hybridization, Genetic
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Nile tilapia
Proteome
Proteomics
RNA-Seq
Transcriptome
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Summary:Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed. •Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents•Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed•miRNAs associated with growth showed a variable expression pattern in crossbreed•Differentially expressed proteins are related to growth by glycolysis pathway
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2021.11.008