Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms

Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it. Methods and Results Antibacterial activity was examined through microdilution assa...

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Bibliographic Details
Published in:Journal of applied microbiology 2022-03, Vol.132 (3), p.1840-1855
Main Authors: Đukanović, Stefana, Ganić, Tea, Lončarević, Branka, Cvetković, Stefana, Nikolić, Biljana, Tenji, Dina, Randjelović, Danijela, Mitić‐Ćulafić, Dragana
Format: Article
Language:English
Subjects:
Aerobic respiration
AFM
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Atomic force microscopy
biofilm
Biofilms
Emodin
Emodin - pharmacology
Gene expression
Humans
Medical equipment
Microbial Sensitivity Tests
Nosocomial infection
Respiration
Staphylococcal Infections - microbiology
Staphylococcus aureus
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Summary:Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it. Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific. Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action. Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.15360