Genetic characterization of ESBL/pAmpC-producing Escherichia coli isolated from forest, urban park and cereal culture soils

• Published in: FEMS microbiology ecology 2021-11, Vol.97 (11)
• Main Authors: Benlabidi, Saloua, Raddaoui, Anis, Achour, Wafa, Hassen, Bilel, Torres, Carmen, Abbassi, Mohamed Salah, Ghrairi, Taoufik
• Format: Article
• Language: English
• Subjects: Animals; Anthropogenic Effects; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; beta-Lactamases - genetics; Edible Grain; Escherichia coli - genetics; Escherichia coli Infections; Forests; Humans; Parks, Recreational; Plasmids; Soil
• ISSN: 1574-6941; 1574-6941
• DOI: 10.1093/femsec/fiab146

ABSTRACT Little is known about the role of forestland and non-fertilized agriculture soils as reservoirs of extended-spectrum beta-lactamase (ESBL) and plasmid-borne AmpC (pAmpC)-producing Escherichia coli isolates. Thus, in the present study, 210 soil samples from various origins (forest of Oued Zen (Ain Drahem), non-agriculture soils from different park gardens in Tunis City, cereal culture soils and home gardens) were investigated to characterize cefotaxime-resistant E. coli isolates. A total of 22 ESBL/pAmpC-producing E. coli were collected, and all harbored variants of the blaCTX-M gene (15 blaCTX-M-1, 5 blaCTX-M-55 and 2 blaCTX-M-15). A total of seven and two isolates harbored also blaEBC and blaDHA-like genes, respectively. Resistances to tetracycline, sulfonamides and fluoroquinolones were encoded by tetA (n = 4)/tetB (n = 12), sul1 (n = 17)/sul2 (n = 19) and aac(6’)-Ib-cr (n = 2)/qnrA (n = 1)/qnrS (n = 1) genes, respectively. A total of seven isolates were able to transfer by conjugation cefotaxime-resistance in association or not with other resistance markers. PFGE showed that ten and two isolates were clonally related (pulsotypes P1 and P2). The 10 P1 isolates had been collected from forestland, cereal culture soils and an urban park garden in Tunis City, arguing for a large spread of clonal strains. Our findings highlight the occurrence of ESBL/pAmpC-E. coli isolates in soils under limited anthropogenic activities and the predominance of CTX-M enzymes that are largely disseminated in E. coli from humans and animals in Tunisia. Forestland, non-fertilized agricultural soils, urban parks and home gardens are reservoirs of ESBL/pAmpC-producing E. coli exhibiting common traits of human and animal isolates.