Lipidomic and metabolomic analysis reveals changes in biochemical pathways for non-small cell lung cancer tissues

Lung cancer represents one of the leading worldwide causes of cancer death, but the pathobiochemistry of this disease is still not fully understood. Here we characterize the lipidomic and metabolomic profiles of the tumor and surrounding normal tissues for 23 patients with non-small cell lung cancer...

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Published in:Biochimica et biophysica acta. Molecular and cell biology of lipids 2022-02, Vol.1867 (2), p.159082, Article 159082
Main Authors: Cífková, Eva, Brumarová, Radana, Ovčačíková, Magdaléna, Dobešová, Dana, Mičová, Kateřina, Kvasnička, Aleš, Vaňková, Zuzana, Šiller, Jiří, Sákra, Lukáš, Friedecký, David, Holčapek, Michal
Format: Article
Language:English
Subjects:
Aged
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Chromatography, Liquid - methods
Female
Humans
Lipid Metabolism
Lipidomics
Lipidomics - methods
Lung cancer
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Mass spectrometry
Metabolism
Metabolomics
Metabolomics - methods
Middle Aged
Tandem Mass Spectrometry - methods
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Summary:Lung cancer represents one of the leading worldwide causes of cancer death, but the pathobiochemistry of this disease is still not fully understood. Here we characterize the lipidomic and metabolomic profiles of the tumor and surrounding normal tissues for 23 patients with non-small cell lung cancer. In total, 500 molecular species were identified and quantified by a combination of the lipidomic shotgun tandem mass spectrometry (MS/MS) analysis and the targeted metabolomic approach using liquid chromatography (LC) – MS/MS. The statistical evaluation includes multivariate and univariate methods with the emphasis on paired statistical approaches. Our research revealed significant changes in several biochemical pathways related to the central carbon metabolism, acylcarnitines, dipeptides as well as the disruption in the lipid metabolism observed mainly for glycerophospholipids, sphingolipids, and cholesteryl esters. •Lipidomic and metabolomic profiles measured for 23 patients tissues with non-small cell lung cancer•500 molecular species were quantified by MS based workflows•Paired statistical evaluation performed by multivariate and univariate methods•Changed pathways related to the central carbon metabolism, acylcarnitines, dipeptides, PL, SL, and CE
ISSN:1388-1981
1879-2618
1879-2618
DOI:10.1016/j.bbalip.2021.159082