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Yeast metabolic engineering for carbon dioxide fixation and its application
[Display omitted] •Developing CO2-fixing yeast is attractive for sustainable industrial bioprocessing.•Yeast metabolic engineering for CO2 fixation and its applications are reviewed.•Recent studies on functional expression of CO2-fixing RuBisCO in yeast are presented.•Construction of CBB cycle in ye...
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Published in: | Bioresource technology 2022-02, Vol.346, p.126349-126349, Article 126349 |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | [Display omitted]
•Developing CO2-fixing yeast is attractive for sustainable industrial bioprocessing.•Yeast metabolic engineering for CO2 fixation and its applications are reviewed.•Recent studies on functional expression of CO2-fixing RuBisCO in yeast are presented.•Construction of CBB cycle in yeast and directed evolution of RuBisCO are summarized.•Potent inorganic carbon-fixing machineries except for RuBisCO are proposed.
As numerous industrial bioprocesses rely on yeast fermentation, developing CO2-fixing yeast strains can be an attractive option toward sustainable industrial processes and carbon neutrality. Recent studies have shown that the expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) in yeasts, such as Saccharomyces cerevisiae and Kluyveromyces marxianus, enables mixotrophic CO2 fixation and production of biofuels. Also, the expression of a synthetic Calvin-Benson-Bassham (CBB) cycle including RuBisCO in Pichia pastoris enables autotrophic growth on CO2. This review highlights recent advances in metabolic engineering strategies to enable CO2 fixation in yeasts. Also, we discuss the potentials of other natural and synthetic metabolic pathways independent of RuBisCO for developing CO2-fixing yeast strains capable of producing value-added biochemicals. |
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ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2021.126349 |