Sequential Cerebrospinal Fluid Sampling in Horses: Comparison of Sampling Times and Two Different Collection Sites

•Two groups of clinically healthy horses were subjected to multiple sequential cerebrospinal fluid (CSF) collections.•Five multiple sequential CSF collections were obtained from the atlanto-occipital (AO) or C1-C2 space.•Chemical and cytologic characteristics were compared between groups and samplin...

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Published in:Journal of equine veterinary science 2022-01, Vol.108, p.103794-103794, Article 103794
Main Authors: Andrade, Danilo Giorgi Abranches de, Cerri, Fabrício Moreira, Barbosa, Giovanna Valverde Magalhães, Basso, Roberta Martins, Takahira, Regina Kiomi, Pantoja, José Carlos de Figueiredo, Oliveira-Filho, José Paes de, Borges, Alexandre Secorun
Format: Article
Language:English
Subjects:
Animals
Atlanto-occipital
Cerebrospinal Fluid Proteins
Cervical
CSF collection
Equine
Erythrocyte Count - veterinary
Horses
Neurologic disease
Reference Values
Specimen Handling - veterinary
Spinal Puncture - veterinary
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Summary:•Two groups of clinically healthy horses were subjected to multiple sequential cerebrospinal fluid (CSF) collections.•Five multiple sequential CSF collections were obtained from the atlanto-occipital (AO) or C1-C2 space.•Chemical and cytologic characteristics were compared between groups and sampling times.•Consecutive AO or C1-C2 CSF collections did not interfere with CSF analysis results. Analysis of the cerebrospinal fluid (CSF) is important as a complementary test in horses with neurologic diseases, and sequential analysis may provide information about the treatment response or evolution and quantitative measures of the CSF drug concentration during treatment. The aim of this study was to compare erythrocyte and nucleated cell counts and protein concentration in multiple CSF samples obtained sequentially from two different puncture sites in clinically healthy horses. Eight and 12 horses, with no evidence of neurologic disease, were subjected to CSF collection from the atlanto-occipital (AO) and C1–C2 spaces, respectively. Cytologic and chemical analyses were performed on the CSF obtained at five sampling times (T1, T2, T3, T4, and T5). Repeated measures models were used to compare the mean erythrocyte count, nucleated cell count, and total protein concentration between the AO and C1–C2 groups at each sampling time. C1–C2 CSF had a significantly higher total protein concentration at T1 and T4 than that of AO CSF. All total protein concentration values remained within the reference interval (
ISSN:0737-0806
1542-7412
DOI:10.1016/j.jevs.2021.103794