Identification of two potential aetiological agents of chronic diarrhoea in an immunocompromised patient in Cuba using conventional and molecular diagnostic techniques

The aetiology of diarrhoea in a patient in Cuba with HIV was investigated. Although molecular diagnostics are still not used in many under-resourced settings, here traditional methods were supported by use of PCR. This approach enabled detection of a dual infection (Cystoisospora belli and Enterocyt...

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Bibliographic Details
Published in:Journal of microbiological methods 2022-01, Vol.192, p.106376-106376, Article 106376
Main Authors: Jerez Puebla, Luis Enrique, Núñez Fernández, Fidel A., Atencio Millán, Iraís, Pérez Avila, Jorge, Fraga Nodarse, Jorge, Cruz Rodríguez, Iredys, Rodríguez Moreno, Laura, Fresco Sampedro, Yanet, Robertson, Lucy J.
Format: Article
Language:English
Subjects:
Adult
Anti-Infective Agents - therapeutic use
Coccidia
Coccidiosis - diagnosis
Coccidiosis - drug therapy
Cuba
Diarrhea - diagnosis
Diarrhea - microbiology
Diarrhea - parasitology
Diarrhoea
Enterocytozoon - genetics
Enterocytozoon - isolation & purification
HIV
HIV Infections - complications
HIV Infections - drug therapy
Humans
Immunocompromised Host
Male
Microscopy
Microsporidia
Microsporidiosis - diagnosis
Microsporidiosis - drug therapy
Molecular assay
Molecular Diagnostic Techniques
Polymerase Chain Reaction
Sarcocystidae - genetics
Sarcocystidae - isolation & purification
Sarcoma, Kaposi - complications
Sarcoma, Kaposi - drug therapy
Trimethoprim, Sulfamethoxazole Drug Combination - therapeutic use
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Summary:The aetiology of diarrhoea in a patient in Cuba with HIV was investigated. Although molecular diagnostics are still not used in many under-resourced settings, here traditional methods were supported by use of PCR. This approach enabled detection of a dual infection (Cystoisospora belli and Enterocytozoon bieneusi), the latter of which was not identified by microscopy with Didier's trichromic staining. •An HIV patient presented with persistent diarrhoea of unknown cause.•Cystoisospora belli was identified by microscopy, but no other pathogens.•Molecular techniques were confirmatory, but also identified Enterocytozoon bieneusi.•The patient responded well to TMP-SMX therapy, but follow-up is advised.•Mixed infections should be considered, even when one pathogen has been identified.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2021.106376