Low-cost apparatus for cigarette smoke exposure in rats

The effects of tobacco smoke on the central nervous system are usually studied with isolated nicotine, ignoring other compounds present in cigarette smoke. The few studies that use in vivo whole-body cigarette smoke exposure are usually performed in expensive commercial apparatus. We presented a fea...

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Bibliographic Details
Published in:Journal of neuroscience methods 2022-01, Vol.366, p.109412, Article 109412
Main Authors: dos Reis Izolan, Lucas, Bandiera, Solange, Pulcinelli, Rianne Remus, Nin, Mauricio Schüler, Almeida, Felipe Borges, Marques, Douglas, Fontella, Fernada Urruth, de Almeida, Roberto Farina, Leal, Mirna Bainy, Gomez, Rosane
Format: Article
Language:English
Subjects:
amino acid
Animals
aspartic acid
cerebrospinal fluid
Cigarette Smoking
Cotinine
glutamate
Nicotine
Rats
tobacco
Tobacco Products
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Summary:The effects of tobacco smoke on the central nervous system are usually studied with isolated nicotine, ignoring other compounds present in cigarette smoke. The few studies that use in vivo whole-body cigarette smoke exposure are usually performed in expensive commercial apparatus. We presented a feasible, safe, and low-cost apparatus for cigarette smoke exposure in rodents. Rats exposed to cigarette smoke in this apparatus showed cotinine levels similar to human active smokers. Additional results showed that cigarette smoke exposure increased glutamate and aspartic acid levels and decreased leucine, isoleucine, ornithine, phenylalanine, and tryptophan levels in the cerebrospinal fluid of rats. Our apparatus is feasible, safe, and costs 67-fold less than a commercial automatized smoking machine. Beyond the low cost, it does not require specialized knowledge for building or maintenance. We concluded that our low-cost apparatus is reliable and reproduces cigarette smoke use in humans. •Our whole-body cigarette smoke exposure system (CSES) is feasible and safe.•The CSES costs 67-fold less than a commercial automatized smoking machine.•Chronic exposure to the CSES reproduces cotinine levels close to an active smoker.•In our CSES carbon monoxide values remained in a secure and below toxic level.•Cigarette smoke increases excitatory amino acids in the cerebrospinal fluid of rats.
ISSN:0165-0270
1872-678X
1872-678X
DOI:10.1016/j.jneumeth.2021.109412