Dual Photoredox Catalysis Strategy for Enhanced Photopolymerization-Based Colorimetric Biodetection

Catalytic redox reactions have been employed to enhance colorimetric biodetection signals in point-of-care diagnostic tests, while their time-sensitive visual readouts may increase the risk of false results. To address this issue, we developed a dual photocatalyst signal amplification strategy that...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2021-12, Vol.13 (48), p.57962-57970
Main Authors: Kim, Seunghyeon, Sikes, Hadley D
Format: Article
Language:English
Subjects:
Applications of Polymer, Composite, and Coating Materials
Biomimetic Materials - chemistry
Catalysis
Colorimetry
Eosine Yellowish-(YS) - analysis
Materials Testing
Methylene Blue - chemistry
Molecular Structure
Oxidation-Reduction
Photochemical Processes
Polymerization
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Summary:Catalytic redox reactions have been employed to enhance colorimetric biodetection signals in point-of-care diagnostic tests, while their time-sensitive visual readouts may increase the risk of false results. To address this issue, we developed a dual photocatalyst signal amplification strategy that can be controlled by a fixed light dose, achieving time-independent colorimetric biodetection in paper-based tests. In this method, target-associated methylene blue (MB+) photocatalytically amplifies the concentration of eosin Y by oxidizing deactivated eosin Y (EYH3–) under red light, followed by photopolymerization with eosin Y autocatalysis under green light to generate visible hydrogels. Using the insights from mechanistic studies on MB+-sensitized photo-oxidation of EYH3–, we improved the photocatalytic efficiency of MB+ by suppressing its degradation. Lastly, we characterized 100- to 500-fold enhancement in sensitivity obtained from MB+-specific eosin Y amplification, highlighting the advantages of using dual photocatalyst signal amplification.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.1c17589