The modulating effect of methoxy-derivatives of 2’-hydroxychalcones on the release of IL-8, MIF, VCAM-1 and ICAM-1 by colon cancer cells

• Published in: Biomedicine & pharmacotherapy 2022-01, Vol.145, p.112428-112428, Article 112428
• Main Authors: Bronikowska, Joanna, Kłósek, Małgorzata, Janeczko, Tomasz, Kostrzewa-Susłow, Edyta, Czuba, Zenon P.
• Format: Article
• Language: English
• Subjects: Adhesion molecules; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Cell Line, Tumor; Chalcone derivatives; Chalcones - administration & dosage; Chalcones - chemistry; Chalcones - pharmacology; Chemoprevention; Colon cancer; Colonic Neoplasms - drug therapy; Cytokines; Dose-Response Relationship, Drug; Humans; Intercellular Adhesion Molecule-1 - metabolism; Interleukin-8 - metabolism; Intramolecular Oxidoreductases - metabolism; Macrophage Migration-Inhibitory Factors - metabolism; Vascular Cell Adhesion Molecule-1 - metabolism
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2021.112428

Colon cancer is one of the leading causes of death in the world. The search for effective and minimally invasive methods of treating colon cancer is the aim of modern medicine. Chalcones and their derivatives have shown an anticancer activity. The aim of the study was to evaluate the effect of methoxy-derivatives of 2’-hydroxychalcones: 2’-hydroxy-3”-methoxychalcone (TJ3), 2’-hydroxy-2”-methoxychalcone (TJ6) and 2’-hydroxy-4”-metoxychalcone (TJ7) at the concentrations of 10 µM and 25 µM on the release of IL-8, MIF, VCAM-1, ICAM-1 by colon cancer SW480 and SW620 cell lines. The cytokines and adhesion molecules were detected using the Bio-Plex Magnetic Luminex Assay and the Bio-Plex Suspension Array System. Our results showed that all tested methoxy-derivatives of 2’-hydroxychalcone compounds significantly reduced ICAM-1 released by SW480 cancer cells. The tested compounds at both concentrations did not significantly affect VCAM-1 released by SW480 and SW620 cancer cell lines. All methoxy-derivatives significantly reduced the concentration of MIF in dose dependent manner on SW480 cells. The TJ3 at the concentration of 25 µM significantly decreased IL-8 secreted by SW480 and SW620 cancer cells. Our results demonstrated that tested methoxy-derivatives of 2’-hydroxychalcones showed modulating effect on colon cancer cells. •The tested compounds could modulating selected mediators released by colon cancer cells.•The soluble factors such as cytokines or adhesion molecules secreted by colon cancer cells determine the microenvironment in which tumor develops.•The tested compounds can be used in cancer chemoprevention through indirect effect in the tumor microenvironment.