MSI1 and HDA6 function interdependently to control flowering time via chromatin modifications

• Published in: The Plant journal : for cell and molecular biology 2022-02, Vol.109 (4), p.831-843
• Main Authors: Xu, Yingchao, Li, Qing, Yuan, Lianyu, Huang, Yisui, Hung, Fu‐Yu, Wu, Keqiang, Yang, Songguang
• Format: Article
• Language: English
• Subjects: Acetylation; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Chromatin; Chromatin - metabolism; Chromatin Immunoprecipitation; Deacetylation; Flowering; FLOWERING LOCUS C; Flowers - genetics; Flowers - metabolism; Gene expression; Gene Expression Regulation, Plant; Gene Silencing; Histone deacetylase; HISTONE DEACETYLASE 6; Histone Deacetylases - genetics; Histone Deacetylases - metabolism; Histone H3; Histones; Histones - metabolism; Immunoprecipitation; Lysine; MADS AFFECTING FLOWERING 4; MADS AFFECTING FLOWERING 5; MADS Domain Proteins - metabolism; Msi1 protein; MULTICOPY SUPRESSOR OF IRA1; Polycomb group proteins; Polycomb Repressive Complex 2 - genetics; Polycomb Repressive Complex 2 - metabolism; Repressor Proteins - metabolism
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/tpj.15596

SUMMARY MULTICOPY SUPPRESSOR OF IRA1 (MSI1) is a conserved subunit of Polycomb Repressive Complex 2 (PRC2), which mediates gene silencing by histone H3 lysine 27 trimethylation (H3K27Me3). Here, we demonstrated that MSI1 interacts with the RPD3‐like histone deacetylase HDA6 both in vitro and in vivo. MSI1 and HDA6 are involved in flowering and repress the expression of FLC, MAF4, and MAF5 by removing H3K9 acetylation but adding H3K27Me3. Chromatin immunoprecipitation analysis showed that HDA6 and MSI1 interdependently bind to the chromatin of FLC, MAF4, and MAF5. Furthermore, H3K9 deacetylation mediated by HDA6 is dependent on MSI1, while H3K27Me3 mediated by PRC2 containing MSI1 is also dependent on HDA6. Taken together, these data indicate that MSI1 and HDA6 act interdependently to repress the expression of FLC, MAF4, and MAF5 through histone modifications. Our findings reveal that the HDA6–MSI1 module mediates the interaction between histone H3 deacetylation and H3K27Me3 to repress gene expression involved in flowering time control. Significance Statement MSI1, a conserved subunit of Polycomb Repressive Complex 2 (PRC2), interacts with the RPD3‐like histone deacetylase HDA6 to repress the expression of FLC, MAF4, and MAF5 through decreasing histone H3 acetylation but increasing H3K27 trimethylation. The MSI1–HDA6 module acts as a bridge between the histone deacetylase complex and PRC2 to mediate the crosstalk between histone H3 deacetylation and H3K27 trimethylation involved in flowering via repressing FLC, MAF4, and MAF5 in Arabidopsis thaliana.