Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia

• Published in: International immunopharmacology 2021-12, Vol.101 (Pt B), p.108366-108366, Article 108366
• Main Authors: Ribeiro, Vanessa Rocha, Romao-Veiga, Mariana, Nunes, Priscila Rezeck, Matias, Mariana Leticia, Peracoli, Jose Carlos, Peracoli, Maria Terezinha Serrao
• Format: Article
• Language: English
• Subjects: Calciferol; Calcitriol; Cell culture; Chemiluminescence; Cytokine; Cytokines; Enzyme-linked immunosorbent assay; Foxp3 protein; GATA-3 protein; Gene expression; Helper cells; Immunoassay; Immunomodulation; Inflammation; Interleukin 10; Interleukin 17; Interleukin 23; Interleukin 6; Lymphocytes; Lymphocytes T; PBMCs; Peripheral blood mononuclear cells; Polarization; Pre-eclampsia; Preeclampsia; Pregnancy; Runx1 protein; Th17; Transcription factors; Treg; Tumor necrosis factor-α; Vitamin D; Vitamin D receptors; γ-Interferon
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2021.108366

[Display omitted] •Vitamin D downregulatesthe sterile inflammation in preeclampsia.•Pregnant women with preeclampsia have lower plasmatic levels of vitamin.•Imbalance between inflammatory and anti-inflammatory T cell subsets in preeclampsia.•Vitamin D polarize T cell subsets to anti-inflammatory and regulatory profiles.•Vitamin D induces an immunomodulatory effect in T cell subsets in preeclamptic women. Vitamin D (VD) is a multifunctional prohormone and low VD status in pregnancy may contribute to the risk of adverse perinatal outcomes, such as preeclampsia (PE). This molecule may modulate the polarization of T cell subsets during gestation. This study evaluated the in vitro immunomodulatory effect of VD [1,25(OH)2D3] on the gene expression of transcription factors and on cytokine production by T cell subsets. Twenty pregnant women with PE and twenty normotensive (NT) pregnant women were studied. Plasma concentration of VD, [25(OH)D3], was evaluated by chemiluminescence. PBMCs from preeclamptic and NT pregnant women were cultured in the absence or presence of VD to determine gene expression of T-bet (Th1), GATA-3 (Th2), RORγt, and RUNX1 (Th17), FoxP3 (regulatory T cell- Treg), and the receptors of VD (VDR) and IL-23 (IL-23R) by quantitative PCR. The concentration of cytokines in the PBMC supernatant culture was determined by cytometric bead array and ELISA immunoassay. The results showed that plasmatic levels of VD were significantly lower in the PE group. The treatment of PBMCs from PE pregnant women with VD induced downregulation of genes related to inflammatory profiles (Th1 and Th17), as well as an increase of the Th2 and Treg profiles. Thus, VD treatment decreased the release of IFN-γ, TNF-α, IL-17, IL-6, and IL-23 while it increased the levels of IL-10 in the PE group. VD induces an immunomodulatory effect in T cell subsets from pregnant women with PE, polarizing these cells to an anti-inflammatory and regulatory profile.