Simultaneous identification and quantification of amino acids in biofluids by reactive 19F-tags

A robust method to identify and quantify amino acids close to physiological conditions by 1D 19F NMR was established. Each 19F-derivatized amino acid has its characteristic chemical-shift profile that is readily identified in the mixture of amino acids or in biofluids including fetal bovine serum an...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-12, Vol.57 (97), p.13154-13157
Main Authors: Ya-Ting, Chen, Li, Bin, Xia-Yan, Li, Jia-Liang, Chen, Chao-Yu, Cui, Hu, Kaifeng, Xun-Cheng, Su
Format: Article
Language:English
Subjects:
Amino acids
Identification methods
NMR
Nuclear magnetic resonance
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A robust method to identify and quantify amino acids close to physiological conditions by 1D 19F NMR was established. Each 19F-derivatized amino acid has its characteristic chemical-shift profile that is readily identified in the mixture of amino acids or in biofluids including fetal bovine serum and cell lysates. The method shows great potential in metabolomics and biochemical analysis.
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc05060e