A Smartphone Optical Device for Point-of-Care Testing of Glucose and Cholesterol Using Ag NPs/UiO-66-NH2‑Based Ratiometric Fluorescent Probe

Point-of-care testing (POCT) with the advantages of simplicity, rapidity, portability, and low-cost is of great importance to improve healthcare, especially in resource-limited settings and home healthcare settings. Moreover, it is a great challenge to quantitative POCT of multiplexed biomarkers wit...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-12, Vol.93 (48), p.16240-16247
Main Authors: Guo, Lan, Chen, Shuai, Yu, Yong-Liang, Wang, Jian-Hua
Format: Article
Language:English
Subjects:
Adaptability
Analytical chemistry
Biomarkers
Chemistry
Cholesterol
Cholesterol oxidase
Detection limits
Diagnostic systems
Fluorescent indicators
Glucose
Glucose oxidase
Health care
Hydrogen peroxide
Low cost
Metabolic disorders
Metabolic syndrome
o-Phenylenediamine
Phenylenediamine
Point of care testing
Portable equipment
Smartphones
Substrates
Target detection
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Point-of-care testing (POCT) with the advantages of simplicity, rapidity, portability, and low-cost is of great importance to improve healthcare, especially in resource-limited settings and home healthcare settings. Moreover, it is a great challenge to quantitative POCT of multiplexed biomarkers within a single accessible assay but provides enhanced diagnostic accuracy and improved diagnostic efficiency. Herein, a smartphone optical device has been designed for POCT of glucose and cholesterol in metabolic syndrome patients using a ratiometric fluorescent sensor. The sensing system of Ag NPs/UiO-66-NH2 and o-phenylenediamine presents a dual-emission response to H2O2 (the main product of glucose and cholesterol catalyzed by glucose oxidase and cholesterol oxidase) on account of the inner filter effect, resulting in an increase in the response of the fluorescence intensity ratio (F 555 nm/F 425 nm) accompanied by a distinguishable color transition from blue to yellow green. After compositing probes with a flexible substrate, the obtained test strip can be integrated with a smartphone-based portable platform to read RGB values for accurate testing of glucose and cholesterol with both detection limits of 10 μmol L–1, which are hundreds of times lower than their concentrations in human serum. With the advantages of low-cost, ease of operation, and broad adaptability, this smartphone optical device holds great potential for portable detection of numerous targets in personalized healthcare and clinical diagnosis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c04126