Highly accurate and label-free discrimination of single cancer cell using a plasmonic oxide-based nanoprobe

The detection of cancer cells at the single-cell level enables many novel functionalities such as next-generation cancer prognosis and accurate cellular analysis. While surface-enhanced Raman spectroscopy (SERS) has been widely considered as an effective tool in a low-cost and label-free manner, how...

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Published in:Biosensors & bioelectronics 2022-02, Vol.198, p.113814-113814, Article 113814
Main Authors: Zhang, Bao Yue, Yin, Pengju, Hu, Yihong, Szydzik, Crispin, Khan, Muhammad Waqas, Xu, Kai, Thurgood, Peter, Mahmood, Nasir, Dekiwadia, Chaitali, Afrin, Sanjida, Yang, Yunyi, Ma, Qijie, McConville, Chris F., Khoshmanesh, Khashayar, Mitchell, Arnan, Hu, Bo, Baratchi, Sara, Ou, Jian Zhen
Format: Article
Language:English
Subjects:
Biosensing Techniques
Colorimetric cell detection
HEK293 Cells
Humans
Leukocytes, Mononuclear
Metal Nanoparticles
Neoplasms - diagnosis
Oxides
Plasmon oxide
Single cell detection
Spectrum Analysis, Raman
Surface enhanced Raman spectroscopy
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Summary:The detection of cancer cells at the single-cell level enables many novel functionalities such as next-generation cancer prognosis and accurate cellular analysis. While surface-enhanced Raman spectroscopy (SERS) has been widely considered as an effective tool in a low-cost and label-free manner, however, it is challenging to discriminate single cancer cells with an accuracy above 90% mainly due to the poor biocompatibility of the noble-metal-based SERS agents. Here, we report a dual-functional nanoprobe based on dopant-driven plasmonic oxides, demonstrating a maximum accuracy above 90% in distinguishing single THP-1 cell from peripheral blood mononuclear cell (PBMC) and human embryonic kidney (HEK) 293 from human macrophage cell line U937 based on their SERS patterns. Furthermore, this nanoprobe can be triggered by the bio-redox response from individual cells towards stimuli, empowering another complementary colorimetric cell detection, approximately achieving the unity discrimination accuracy at a single-cell level. Our strategy could potentially enable the future accurate and low-cost detection of cancer cells from mixed cell samples. •Plasmonic oxide (PO) exhibits both SERS and colorimetric detection capabilities.•PO is highly biocompatible and ultra-sensitive towards cellular redox reactions.•The maximum accuracy of single cancer cell SERS detection is consistently above 90%.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2021.113814