Application of HRP-streptavidin bionanoparticles for potentiometric biotin determination

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2022-04, Vol.144, p.107993, Article 107993
• Main Authors: Büyüktiryaki, Sibel, Yazıcı, Burcu, Ersöz, Arzu, Say, Rıdvan, Özkütük, Ebru Birlik
• Format: Article
• Language: English
• Subjects: Affinity interaction; Biosensing Techniques - methods; Biosensors; Biotin; Biotin - chemistry; Biotin determination; Dietary supplements; Humans; Limit of Detection; Low concentrations; Potentiometric sensor; Potentiometry - instrumentation; Potentiometry - methods; Regression coefficients; Reproducibility of Results; Sensors; Streptavidin; Streptavidin - chemistry
• ISSN: 1567-5394; 1878-562X; 1878-562X
• DOI: 10.1016/j.bioelechem.2021.107993

[Display omitted] •A potentiometric biotin sensor was reported using nano-HRP-streptavidin conjugates.•Covalent and photosensitive crosslinking conjugation was performed.•The potentiometric sensor exhibited high sensitivity, stability, and selectivity.•Good recoveries were obtained for analysis of infant formula and supplement.•Developed biosensor able to detect biotin with LOD of 0.3 10−15M. Biotin is widely used in infant formula to prevent biotin deficiency of newborn babies and in beauty products as nutritional supplements for coenzymatic functions and having strong nails, shiny hair, and skin over the last few years. There is a need for the development of a fast, simple and reusable assay method to perform biotin determination at very low concentrations. Biotin determination has achieved with a prepared potentiometric biotin sensor that has a very wide concentration range (10−15M–10−7M) and a lower detection limit (0.3 10−15M) with a very good regression coefficient (0.9925). A quick response (7 min), good accuracy (recovery 100.4–103.7%), reproducible, reusable (10 times), and long-term stability (3 months) have been obtained using the prepared potentiometric sensor. The obtained results have proved that the prepared potentiometric sensor can be used for biotin determination in real samples.