The analgesic activities of total alkaloids of the ethnic medicine Cynanchum komarovii Al. Iljinski

• Published in: Journal of ethnopharmacology 2022-03, Vol.285, p.114861-114861, Article 114861
• Main Authors: Wang, Ruizhou, Tao, Li, Lu, Qiang, Hao, Fusheng, Zhao, Shipeng, Ma, Yan, Han, Lu, Bai, Changcai
• Format: Article
• Language: English
• Subjects: Activation of astrocytes; Alkaloids; Alkaloids - chemistry; Alkaloids - pharmacology; Alkaloids - toxicity; Analgesic activities; Analgesics - chemistry; Analgesics - pharmacology; Analgesics - toxicity; Animals; Cynanchum - chemistry; Cynanchum komarovii; Dose-Response Relationship, Drug; Female; Humans; Inflammation - drug therapy; Inflammatory mediators; Kidney - drug effects; Lethal Dose 50; Liver - drug effects; Male; Medicine, Traditional; Mice; Mice, Inbred ICR; Pain - drug therapy; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - toxicity; Plant Leaves - chemistry; Plant Roots - chemistry; Plant Stems - chemistry; Rats; Time Factors
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2021.114861

Cynanchum komarovii Al. Iljinski is a ethnomedicinal herb and this ethno-medicine is used mainly to treat arthritis, toothache, reducing phlegm, relieving cough. Total alkaloids of Cynanchum komarovii Al. Iljinski (TACKI) is the main active compound of Cynanchum komarovii Al. Iljinski. Previous investigations have revealed that TACKI can significantly inhibit rat foot swelling caused by carrageenan; it has a significant inhibitory effect on granulation tissue proliferation. Pharmacology study showed that Cynanchum komarovii Al. Iljinski has analgesia, anti-inflammatory, antibacterial, anti-tumor, relieving cough and relieving asthma. However, there is no any investigation on the mechanism of analgesia and anti-inflammation. To clarify the analgesic effect and material basis of Cynanchum komarovii Al. Iljinski, determine the analgesic effect of TACKI, and provide experimental data support for its traditional application in the treatment of various pains. TACKI were prepared by the traditional acid extraction and alkaline precipitation method, and TACKI was analyzed through classic animal models of acute antinociceptive animal models and chronic antinociceptive. Evaluation of analgesic effects, and preliminary discussion of the mechanism of its analgesic effects were performed in this work. Acute toxicity experiments showed that the LD50 of TACKI mice was 2960.88 mg/kg, and symptoms of poisoning appeared. Patholog of liver and kidney studies have shown that TACKI reduces eosinophils and increases basophils in kidney glomeruli. In the study of analgesic effects, TACKI had analgesic activity through the PWL, formalin test, and acetic acid writhing test. In the chronic inflammatory antinociceptive study, the latency of the withdrawal reflex in the TACKI group was prolonged, and the mechanical withdrawal reflex threshold was significantly increased. The protein expression of NMDA, GFAP and Iba-1 in rat brain tissue can be reduced significantly byTACKI. Meanwhile, the content of TNF-α and IL-6 in rat brain tissue is reduced. TACKI has a significant analgesic activities. It may be related to inhibiting the activation of astrocytes and reducing the content of inflammatory mediators. [Display omitted]