Distribution of cholinergic neurons in the brains of a lar gibbon and a chimpanzee

• Published in: Anatomical record (Hoboken, N.J. : 2007) N.J. : 2007), 2022-06, Vol.305 (6), p.1516-1535
• Main Authors: Williams, Victoria M., Bhagwandin, Adhil, Swiegers, Jordan, Bertelsen, Mads F., Hård, Therese, Sherwood, Chet C., Manger, Paul R.
• Format: Article
• Language: English
• Subjects: acetylcholine; Acetyltransferase; Animals; ape; Brain - metabolism; brain evolution; Cerebral cortex; choline acetyltransferase; Choline O-acetyltransferase; Choline O-Acetyltransferase - metabolism; Cholinergic Agents; Cholinergic Neurons - metabolism; Cholinergic transmission; Dorsal horn; Dorsal tegmental nucleus; Hylobates; Hylobates lar; Immunohistochemistry; Laterodorsal tegmental nucleus; Locomotion; Mammals; Medulla oblongata; Monkeys & apes; Pan troglodytes; Pedunculopontine tegmental nucleus; Primates; Pyramidal cells; Spinal cord
• ISSN: 1932-8486; 1932-8494
• DOI: 10.1002/ar.24844

Using choline acetyltransferase immunohistochemistry, we describe the nuclear parcellation of the cholinergic system in the brains of two apes, a lar gibbon (Hylobates lar) and a chimpanzee (Pan troglodytes). The cholinergic nuclei observed in both apes studied are virtually identical to that observed in humans and show very strong similarity to the cholinergic nuclei observed in other primates and mammals more generally. One specific difference between humans and the two apes studied is that, with the specific choline acetyltransferase antibody used, the cholinergic pyramidal neurons observed in human cerebral cortex were not labeled. When comparing the two apes studied and humans to other primates, the presence of a greatly expanded cholinergic medullary tegmental field, and the presence of cholinergic neurons in the intermediate and dorsal horns of the cervical spinal cord are notable variations of the distribution of cholinergic neurons in apes compared to other primates. These neurons may play an important role in the modulation of ascending and descending neural transmissions through the spinal cord and caudal medulla, potentially related to the differing modes of locomotion in apes compared to other primates. Our observations also indicate that the average soma volume of the neurons forming the laterodorsal tegmental nucleus (LDT) is larger than those of the pedunculopontine nucleus (PPT) in both the lar gibbon and chimpanzee. This variability in soma volume appears to be related to the size of the adult derivatives of the alar and basal plate across mammalian species.