Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response

Vascular calcification is a major complication of chronic renal failure, which has been identified as an active process partly driven by osteogenic transition of vascular smooth muscle cells (VSMCs). Aspirin could prevent cardiomyocyte damage by inducing heat shock response. This study investigates...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceutical biology 2022-12, Vol.60 (1), p.17-24
Main Authors: Shen, Quanquan, Chen, Qian, Liu, Yang, Xue, Xiang, Shen, Xiaogang, He, Qiang, Wang, Guokun, Han, Fei
Format: Article
Language:English
Subjects:
Animals
Aorta
Aorta - cytology
Aorta - drug effects
Aspirin
Aspirin - pharmacology
Calcification
Calcification (ectopic)
Calcium (intracellular)
Cardiomyocytes
Cbfa-1 protein
Cell culture
Heat shock
heat shock protein 70
heat shock protein 90
heat shock transcription factor 1
Heat-Shock Response - drug effects
HSF1 protein
HSP70 Heat-Shock Proteins - metabolism
Hsp70 protein
HSP90 Heat-Shock Proteins - metabolism
Hsp90 protein
Intracellular
Male
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - drug effects
Myocytes, Smooth Muscle - drug effects
Myocytes, Smooth Muscle - pathology
Osteoblasts
Platelet Aggregation Inhibitors - pharmacology
Rats
Rats, Sprague-Dawley
Renal failure
siRNA
Smooth muscle
Vascular Calcification - drug therapy
Vascular smooth muscle cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vascular calcification is a major complication of chronic renal failure, which has been identified as an active process partly driven by osteogenic transition of vascular smooth muscle cells (VSMCs). Aspirin could prevent cardiomyocyte damage by inducing heat shock response. This study investigates the effect of aspirin on alleviating VSMC calcification. An in vitro VSMC calcification model was established by 10-day calcification induction in osteogenic medium. VSMCs were grouped as following: control group (normal medium), calcified group (osteogenic medium) and treated group (osteogenic medium with 1 or 4 mmol/L aspirin). VSMC calcification was evaluated by calcified nodules formation, intracellular calcium concentration and osteoblastic marker (OPN and Runx2) expression. After 10-day culture, the intracellular calcium concentration in calcified group was significantly higher than that in control group (1.16 ± 0.04 vs. 0.14 ± 0.01 μg/mg, p 
ISSN:1388-0209
1744-5116
DOI:10.1080/13880209.2021.2007268