Production, immobilization and application of invertase from new wild strain Cunninghamella echinulata PA3S12MM

• Published in: Journal of applied microbiology 2022-04, Vol.132 (4), p.2832-2843
• Main Authors: Rasbold, Letícia Mara, Delai, Vitória Maciel, Cruz Kerber, Caroline Marlene, Simões, Márcia Regina, Heinen, Paulo Ricardo, Conceição Silva, José Luis, Cássia Garcia Simão, Rita, Kadowaki, Marina Kimiko, Maller, Alexandre
• Format: Article
• Language: English
• Subjects: agro‐industrial waste; Alginates; Alginic acid; beta-Fructofuranosidase - metabolism; Calcium alginate; Calcium phosphates; Chitosan; Cunninghamella - metabolism; Cunninghamella echinulata; Enzyme Stability; Enzymes; Enzymes, Immobilized; Fixed bed reactors; Fixed beds; Fructose; Gelatin; glutaraldehyde‐activated chitosan support; Hydrogen-Ion Concentration; Immobilization; Industrial applications; Invertase; inverted sugar; pH effects; Sucrose; Temperature; Thermal resistance; Thermal stability; β‐fructofuranosidase
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.15394

Aims The objective of this study was to determine the best conditions to produce invertase by Cunninghamella echinulata PA3S12MM and to immobilize and apply the enzyme. Methods and Results The maximum production was verified in 8 days of cultivation at 28°C supplemented with 10 g L–1 apple peel, reaching 1054.85 U ml–1. The invertase was purified from the DEAE‐Sephadex column. The derivative immobilized in alginate‐gelatin‐calcium phosphate showed reusability >50% for 19 cycles. The derivative immobilized in glutaraldehyde‐chitosan showed greater thermostability and at a different pH. The hydrolysis of 15 ml of sucrose 500 g L–1 in a fixed bed reactor (total volume of 31 ml) produced 24.44 µmol min–1 of glucose and fructose at a residence time of 30 min and a conversion factor of 0.5. Conclusions The new wild strain C. echinulata PA3S12MM presents high invertase production in medium supplemented with an agro‐industrial residue and the immobilized enzyme showed high thermal stability and resistance at a different pH. Significance and Impact of the Study The fungus C. echinulata PA3S12MM is an excellent producer of invertases in Vogel medium supplemented with apple peel. The enzyme is promising for industrial application since it has good performance in reusability and inverted sugar production.