Microinflammation in the intestinal mucosa and symptoms of irritable bowel syndrome

• Published in: Journal of gastroenterology 2022-02, Vol.57 (2), p.62-69
• Main Authors: Yoshimoto, Takanori, Oshima, Tadayuki, Huang, Xinyi, Tomita, Toshihiko, Fukui, Hirokazu, Miwa, Hiroto
• Format: Article
• Language: English
• Subjects: Abdominal Surgery; Antibodies; Biopsy; Case-Control Studies; Chemokines; Colorectal Surgery; CXCL11 protein; Cytokines; Diarrhea; Diarrhea - complications; Duodenum; Duodenum - pathology; Gastroenterology; Hepatology; Humans; Ileum; Ileum - pathology; Immune response; Infiltration; Inflammation; Intestinal Mucosa - pathology; Intestine; Irritable bowel syndrome; Irritable Bowel Syndrome - diagnosis; Lymphocytes; Mast cells; Medicine; Medicine & Public Health; Mucosa; Original Article—Alimentary Tract; Polymerase chain reaction; Reverse transcription; RNA; Small intestine; Surgical Oncology; Viral antibodies
• ISSN: 0944-1174; 1435-5922
• DOI: 10.1007/s00535-021-01838-4

Background Potential etiological mechanisms of irritable bowel syndrome (IBS) have been reported, and emerging data suggest that immune activation is present in a major subset of IBS, especially in those with diarrhea. Intestinal mucosal mast cell and intraepithelial lymphocyte (IEL) infiltration and related factors were examined in patients with IBS. In addition, the correlations of symptoms and micro-inflammation were assessed. Methods Intestinal biopsy specimens were obtained from patients with IBS and controls. Mast cells and IELs were stained with specific antibodies. The mRNA levels of cytokines and chemokines were assessed by quantitative reverse transcription polymerase chain reaction. Results Infiltration of mast cells in the duodenum was significantly higher in IBS patients than in control subjects. The infiltration of IELs was higher in the duodenum and terminal ileum of IBS patients compared to the control subjects. The numbers of duodenal and ileal IELs were significantly correlated. The number of IELs but not mast cells in the duodenum and terminal ileum was significantly correlated with diarrhea frequency in control subjects and IBS patients. The expression level of the chemotactic chemokine CXCL11 was significantly higher in the duodenum of IBS patients. Conclusion Duodenal mast cells and IELs were increased in IBS patients. In addition, a positive correlation was found between the number of duodenal and ileal IELs and the frequency of diarrhea. Given that the present study was strictly observational, further studies are needed to clarify the pathophysiological functions associated with micro-inflammation in IBS.