Comparative Study of the γH2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids

• Published in: Bulletin of experimental biology and medicine 2021-12, Vol.172 (2), p.245-249
• Main Authors: Rodneva, S. M., Osipov, A. A., Guryev, D. V., Tsishnatti, A. A., Fedotov, Y. А., Yashkina, E. I., Vorobyova, N. Y., Maksimov, A. A., Kochetkov, O. A., Samoylov, A. S., Osipov, A. N.
• Format: Article
• Language: English
• Subjects: Alanine; Amino acids; Amino Acids - chemistry; Amino Acids - pharmacology; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cells, Cultured; Chromatin; Comparative analysis; Culture Media - chemistry; Culture Media - pharmacology; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded - radiation effects; DNA damage; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - pathology; Fibroblasts - radiation effects; Histones - genetics; Histones - metabolism; Humans; Internal Medicine; Laboratory Medicine; Lung - drug effects; Lung - metabolism; Lung - pathology; Lung - radiation effects; Neoplastic Stem Cells - drug effects; Neoplastic Stem Cells - radiation effects; Pathology; Proline; Radioactivity; Thymidine; Thymidine - chemistry; Thymidine - pharmacology; Tritium; Tritium - chemistry; Tritium - pharmacology
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-021-05370-6

We compared the formation of γ H2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3 H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of γ H2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of 3 H-thymidine was more than 2-fold higher than under the influence of 3 H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with 3 H-labeled amino acids showed that 3 H-alanine produced more pronounced effect that 3 H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins.