Kinetics and molecular mechanism of enhanced fluoranthene biodegradation by co-substrate phenol in co-culture of Stenotrophomonas sp. N5 and Advenella sp. B9

• Published in: Environmental research 2022-04, Vol.205, p.112413-112413, Article 112413
• Main Authors: Changmei, Li, Gengrui, Wei, Haizhen, Wu, Yuxiao, Wang, Shuang, Zhu, Chaohai, Wei
• Format: Article
• Language: English
• Subjects: Biodegradation; Biodegradation, Environmental; Coculture Techniques; Differentially expressed genes; DNA repair; Fluoranthene; Fluorenes; Kinetics; Phenol; Phenol - metabolism; Phenols - analysis; Polycyclic Aromatic Hydrocarbons - analysis; Stenotrophomonas - metabolism; Transcriptome analysis
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2021.112413

Polycyclic aromatic hydrocarbons (PAHs) and phenol are persistent pollutants that coexist in coking wastewater (CWW). Fluoranthene (Flu) is the predominant PAH species in the CWW treatment system. Our work emphasized on distinguishing the effects of phenol on Flu biodegradation by co-culture of Stenotrophomonas sp. N5 and Advenella sp. B9 and illustrated the molecular mechanisms. Results showed Flu biodegradation by co-culture was enhanced by phenol. According to the first-order degradation kinetic analysis of Flu, phenol significantly increased the biodegradation rate constant and shortened the half-life of Flu. Transcriptome analysis pointed out the up-regulation of DNA repair activity and 3717 significantly differentially expressed genes (DEGs), were triggered by 800 mg/L phenol. GO enrichment analysis suggested these DEGs are mainly concentrated in biochemical processes such as metal ion binding and alpha-amino acid biosynthesis, which are closely associated with Flu biodegradation, indicating that phenol promotes DNA repair activity and reduces Flu genotoxicity. qRT-PCR was performed to detect the gene expression of aromatic ring-opening dioxygenase. Combined with transcriptome analysis, the qRT-PCR results suggested phenol did not induce the expression of related PAHs-degrading enzymes. RNA extraction and microbial growth curves of COC and COC + Ph provided further evidence that phenol serves as co-substrate which increases biomass and the concentration of degrading enzymes, therefore promoting the Flu degradation. •Phenol significantly facilitated the degradation of fluoranthene.•Phenol was served as the growth substrate to increase biomass.•DNA repair activity was enhanced by addition of phenol.•3717 differentially expressed genes was triggered by phenol.•Gene expression regulation by phenol promoted degradation of fluoranthene.