Ia EPSPs in rat spinal motoneurons are potentiated after a 5-week whole body vibration

• Published in: Journal of applied physiology (1985) 2022-01, Vol.132 (1), p.178-186
• Main Authors: Krutki, Piotr, Mrówczyński, Włodzimierz, Celichowski, Jan, Bączyk, Marcin
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Electrical stimuli; Excitation; Excitatory Postsynaptic Potentials; Male; Membrane potential; Membranes; Motor Neurons; Muscle spindles; Muscle strength; Muscle, Skeletal; Muscles; Physical training; Potentiation; Rats; Rats, Wistar; Rehabilitation; Sensory neurons; Spinal Cord; Synapses; Synaptic plasticity; Training; Vibration
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00519.2021

Whole body vibration (WBV) is often applied as an alternative method for strength training or to prevent muscle force decrease. In this study, we evaluated the influence of WBV on Ia monosynaptic input from muscle spindles because the tonic vibration reflex is responsible for the enhancement of muscle activity observed after WBV. The aim was to investigate whether repeated activation of muscle spindles during WBV may result in altered synaptic excitation of motoneurons. WBV was performed on adult male Wistar rats, 5 days/wk, for 5 wk, and each daily session consisted of four 30-s runs of vibration at 50 Hz. Fast-type medial gastrocnemius motoneurons were investigated intracellularly in deeply anesthetized animals in the experimental ( = 7, 34 motoneurons) and control ( = 7, 32 motoneurons) groups. Monosynaptic Ia excitatory postsynaptic potentials (EPSPs) were evoked by electrical stimulation of afferent fibers from the synergistic lateral gastrocnemius and soleus muscles. Data were analyzed using a mixed linear model. The central latencies of EPSPs were 0.45-0.9 ms with no differences in the mean values between the analyzed groups ( = 0.291). WBV induced an increase of the mean EPSP amplitude by 28% ( = 0.025), correlated with the resting membrane potential and input resistance, and a shortening of the mean EPSP rise time by 11% ( = 0.012). The potentiation of synaptic excitation of motoneurons was not accompanied by changes of passive membrane properties, pointing to synaptic plasticity. This indicates that WBV may support rehabilitation or training processes aimed at increasing muscle strength on the basis of increased motoneuronal drive. The study provides new information on neuronal plasticity following repeatedly exerted mechanical loading. We demonstrate in electrophysiological experiments on rat lumbar motoneurons that low-volume whole body vibration applied systematically for 5 wk potentiates synaptic excitation from primary muscle afferents. The adaptive changes are expressed by higher amplitudes and shorter rise times of monosynaptic EPSPs evoked in motoneurons of the vibrated group compared with the control.