Dynamic changes in rhizosphere fungi in different developmental stages of wheat in a confined and isolated environment

• Published in: Applied microbiology and biotechnology 2022, Vol.106 (1), p.441-453
• Main Authors: Sun, Ruxin, Yi, Zhihao, Fu, Yuming, Liu, Hong
• Format: Article
• Language: English
• Subjects: Ascomycota; Bioinformatics; Biomedical and Life Sciences; Biotechnology; Chytridiomycota; Clustering; Community composition; Community structure; Composition; Crop production; Development; Developmental stages; Environmental aspects; Environmental Biotechnology; Flowering; Function analysis; Fungi; Fungi - genetics; Life Sciences; Microbial activity; Microbial Genetics and Genomics; Microbiology; Microorganisms; Mycorrhizae; Physiological aspects; Plant Roots; Plants; Rhizosphere; Seedlings; Soil Microbiology; Species composition; Structure-function relationships; Triticum; Wheat
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-021-11698-w

As the core food crop of a bioregenerative life support system (BLSS), wheat is susceptible to pathogen infection due to the lack of effective microbial communities in the confined and isolated environment. Therefore, a thorough understanding of the dynamic changes in wheat rhizosphere fungi is of great significance for improving wheat production and ensuring the stability of the BLSS. In the current study, we collected samples of rhizosphere fungi in the four growth stages of wheat grown in the “Lunar Palace 365” experiment. We employed bioinformatics methods to analyze the samples’ species composition characteristics, community network characteristics, and FUNGuild function analysis. We found that the species composition of rhizosphere fungi in the wheat at the tillering stage changed greatly in the closed and isolated environment, while the species composition in the seedling, flowering, and mature stage were relatively stable. The results of the FUNGuild function analysis showed that the functions of rhizosphere fungi changed during wheat development. The rhizosphere fungal community was centered on Ascomycota , Mortierellomycota , and Chytridiomycota , and the community showed the characteristics of a “small world” arrangement. The stage of wheat seedlings is characterized by a greater abundance, diversity, and complexity of the network of interactions in the rhizosphere mycorrhiza community, while the tillering stage exhibited a greater clustering coefficient. Based on the changes in species composition, guild function regulation, and community structure differences of the wheat rhizosphere fungi in the BLSS, our study identified the critical fungal species during wheat development, providing a reference for ensuring the health and yield of plants in the BLSS system. Key points • The diversity, composition, FUNguild, and network structure of rhizosphere fungi were analyzed. • Ascomycota, Mortierellomycota, and Chytridiomycota were the center of the rhizosphere fungal community network. •  The effects of different wheat developmental stages on the community composition, function, and network structure of rhizosphere fungi were examined.