Sabulicella rubraurantiaca gen. nov., sp. nov., a new member of the family Acetobacteraceae, isolated from desert soil

• Published in: Archives of microbiology 2022-01, Vol.204 (1), p.1-1, Article 1
• Main Authors: Lin, Zhi-Liang, Li, Shuai, Wei, Qi-Chuang, Lian, Wen-Hui, Lu, Chun-Yan, Xu, Lu, Huang, Li-Nan, Dong, Lei, Li, Wen-Jun
• Format: Article
• Language: English
• Subjects: Acetobacteraceae; Bacterial Typing Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Cell Biology; Desert soils; Deserts; DNA, Bacterial - genetics; Ecology; Fatty acids; Gene sequencing; Lecithin; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; Original Paper; pH effects; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phylogenetics; Phylogeny; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sandy soils; Sequence Analysis, DNA; Sodium chloride; Soil; Ubiquinone
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02604-z

Strain SYSU D01096 T was isolated from a sandy soil sample collected from Gurbantunggut Desert in Xinjiang, PR China. Phylogenetic analysis of the nearly full-length 16S rRNA gene sequence revealed that strain SYSU D01096 T belonged to the family Acetobacteraceae and was closest to Rubritepida flocculans DSM 14296 T (96.0% similarity). Cells of strain SYSU D01096 T were observed to be non-motile, short rod-shaped and Gram-staining negative. The colonies were observed to be translucent, reddish orange, circular, convex and smooth. Growth occurred at 15–37 °C (optimum, 28–30 °C), pH 4.0–8.0 (optimum, pH 7.0) and 0–0.5% NaCl (w/v; optimum, 0%) on Reasoner’s 2A medium. The predominant ubiquinone was identified as ubiquinone 9 and the major fatty acids were Summed Feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ) and C 16:0 . The polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), one unidentified phospholipid (PL), three unidentified aminolipids (AL1-3) and one unidentified aminophospholipid (APL). The genomic DNA G + C content was 69.1%. Phylogenetic tree based on 16S rRNA gene sequences indicated strain SYSU D01096 T represented an individual lineage in the family Acetobacteraceae , which was supported by 30 core gene-based phylogenomic tree. Based on the multi-analysis including physiological, chemotaxonomic and phylogenetic comparison, strain SYSU D01096 T was proposed to represent a novel species of a novel genus, named Sabulicella rubraurantiaca gen. nov., sp. nov., within the family Acetobacteraceae . The type strain is SYSU D01096 T (= CGMCC 1.8619 T  = KCTC 82268 T  = MCCC 1K04998 T ).