Development of a recombinase polymerase amplification assay for rapid detection of Streptococcus suis type 2 in nasopharyngeal swab samples

Streptococcus suis serotype 2 (SS2), an emerging zoonotic pathogen, may induce severe infections and symptoms manifested as septicemia, meningitis and even death both in human and pigs. The aim of this article was to develop a new methodology as real-time recombinase polymerase amplification (RT-RPA...

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Published in:Diagnostic microbiology and infectious disease 2022-02, Vol.102 (2), p.115594-115594, Article 115594
Main Authors: Jiang, Xiaowu, Zhu, Lexin, Zhan, Dongbo
Format: Article
Language:English
Subjects:
Genetic Variation
Genotype
Humans
Nasopharynx - microbiology
Nucleic Acid Amplification Techniques - methods
Rapid detection
Real-Time Polymerase Chain Reaction - methods
Recombinases - genetics
Reproducibility of Results
RT-RPA
Sensitivity and Specificity
Streptococcus suis - genetics
Streptococcus suis - isolation & purification
Streptococcus suis type 2
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Summary:Streptococcus suis serotype 2 (SS2), an emerging zoonotic pathogen, may induce severe infections and symptoms manifested as septicemia, meningitis and even death both in human and pigs. The aim of this article was to develop a new methodology as real-time recombinase polymerase amplification (RT-RPA) assay targeting cps2J gene for the detection of SS2 (or SS1/2). The sensitivity and reproducibility of RT-RPA results were evaluated and compared with a real-time quantitative PCR (RT-qPCR). The established RT-RPA reaction could be completed in 20 minutes with distinguishable specificity against the predominant S. suis infection serotypes of 3, 4, 5, 7, 9, 14, and 31. Lower detection limit for RT-RPA was 102 genomic DNA copies per reaction. The specimen performance of RT-RPA was tested in nasopharyngeal swab samples with the sensitivity and specificity as 97.5% and 100%, respectively. Thus, this RT-RPA method is a rapid and potential molecular diagnostic tool for SS2 detection.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2021.115594