Dendrobium fimbriatum Hook polysaccharide ameliorates dextran-sodium-sulfate-induced colitis in mice via improving intestinal barrier function, modulating intestinal microbiota, and reducing oxidative stress and inflammatory responses

• Published in: Food & function 2022-01, Vol.13 (1), p.143-160
• Main Authors: Wang, Yu-Jing, Li, Qiang-Ming, Zha, Xue-Qiang, Luo, Jian-Ping
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - chemistry; Antioxidants - pharmacology; Bacteria; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - metabolism; Colon; Cytokines; Dendrobium; Dendrobium - chemistry; Dextran; Dextran Sulfate - adverse effects; Dextrans; Disease Models, Animal; Gastrointestinal Microbiome - drug effects; Helper cells; Homeostasis; Inflammation - metabolism; Inflammatory bowel disease; Inflammatory bowel diseases; Intestinal microflora; Intestine; Lymphocytes T; Male; Metabolism; Mice; Mice, Inbred C57BL; Microbiota; NF-κB protein; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Signal Transduction - drug effects; Signaling; Sodium; Sulfates; Ulcerative colitis; Weight loss; Western blotting
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d1fo03003e

The ameliorative effect of polysaccharide (cDFPW1) on ulcerative colitis (UC) was investigated using a dextran-sodium-sulfate-induced (DSS-induced) mouse model in the present study. The results showed that cDFPW1 effectively improved colitis in mice by ameliorating weight loss, disease activity index (DAI) and colonic pathological damage, and by protecting the intestinal barrier function integrity. Moreover, cDFPW1 modulated the composition and metabolism of intestinal microbiota through enhancing , and , and reducing , and in colitis mice. Notably, cDFPW1 significantly restored the homeostasis of Th17/regulatory T (Treg) cells and the expression of specific cytokines. Western blotting of colon tissues showed that cDFPW1 markedly up-regulated the expression of Nrf2 and inhibited the phosphorylation of NF-κB signaling. These results indicated that cDFPW1 possesses the potential of improving UC and its effect on palliating colitis may be connected with the regulation of Nrf2/NF-κB signaling.