Detection of glioblastoma multiforme using quantitative molecular magnetic resonance imaging based on 5-aminolevulinic acid: in vitro and in vivo studies

Objectives We demonstrated a novel metabolic method based on sequential administration of 5-aminolevulinic acid (ALA) and iron supplement, and ferric ammonium citrate (FAC), for glioblastoma multiforme (GBM) detection using R 2 ’ and quantitative susceptibility mapping (QSM). Materials and methods I...

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Published in:Magma (New York, N.Y.) N.Y.), 2022-02, Vol.35 (1), p.3-15
Main Authors: Ebrahimpour, Anita, Tirgar, Fatemeh, Hajipour-Verdom, Behnam, Abbasi, Ardeshir, Hadjighassem, Mahmoudreza, Abdolmaleki, Parviz, Hosseindoost, Saereh, Javadi, Seyed Amir Hossein, Hashemi, Hassan, Foroushani, Abbas Rahimi, Alam, Nader Riyahi, Khoobi, Mehdi
Format: Article
Language:English
Subjects:
Aminolevulinic Acid
Animals
Basic Science - Molecular and cellular imaging
Biomedical Engineering and Bioengineering
Computer Appl. in Life Sciences
Glioblastoma - diagnostic imaging
Health Informatics
Imaging
Iron
Magnetic Resonance Imaging - methods
Medicine
Medicine & Public Health
Radiology
Rats
Rats, Wistar
Research Article
Solid State Physics
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Summary:Objectives We demonstrated a novel metabolic method based on sequential administration of 5-aminolevulinic acid (ALA) and iron supplement, and ferric ammonium citrate (FAC), for glioblastoma multiforme (GBM) detection using R 2 ’ and quantitative susceptibility mapping (QSM). Materials and methods Intra-cellular iron accumulation in glioblastoma cells treated with ALA and/or FAC was measured. Cell phantoms containing glioblastoma cells and Wistar rats bearing C 6 glioblastoma were imaged using a 3 T MRI scanner after sequential administration of ALA and FAC. The relaxivity and QSM analysis were performed on the images. Results The intra-cellular iron deposition was significantly higher in the glioma cells with sequential treatment of ALA and FAC for 6 h compared to those treated with the controls. The relaxivity and magnetic susceptibility values of the glioblastoma cells and rat brain tumors treated with ALA + FAC (115 ± 5 s-1 for R 2 ’, and 0.1 ± 0.02 ppm for magnetic susceptibility) were significantly higher than those treated with the controls (55 ± 18 (FAC), 45 ± 15 (ALA) s-1 for R 2 ’, p  
ISSN:0968-5243
1352-8661
DOI:10.1007/s10334-021-00978-1