Intake of high-purity insoluble dietary fiber from Okara for the amelioration of colonic environment disturbance caused by acute ulcerative colitis

• Published in: Food & function 2022-01, Vol.13 (1), p.213-226
• Main Authors: Lyu, Bo, Wang, Yi, Fu, Hongling, Li, Jiaxin, Yang, Xiaoqing, Shen, Yue, Swallah, Mohammed Sharif, Yu, Ziyue, Li, Yang, Wang, Huan, Yu, Hansong, Jiang, Lianzhou
• Format: Article
• Language: English
• Subjects: Animals; Bioinformatics; Colitis, Ulcerative - metabolism; Colon; Colon - drug effects; Dietary fiber; Dietary Fiber - pharmacology; Dietary intake; Disease Models, Animal; Fatty acids; Fatty Acids, Volatile - metabolism; Female; Gastrointestinal Microbiome - drug effects; Glycine max - chemistry; Inflammatory bowel disease; Inflammatory bowel diseases; Mice; Mice, Inbred C57BL; Okara; Purity; rRNA 16S; Shigella; Ulcerative colitis
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d1fo02264d

High-purity insoluble dietary fiber from okara (okara-HPIDF) is a raw material with a potentially positive effect on colon health. However, the mechanisms of the effect are far from clear. In this study, okara-HPIDF and low-purity dietary fiber from okara (okara-LPDF) were fed to C57BL/6 mice with acute ulcerative colitis induced by DSS. The levels of inflammatory factors, bacterial 16S rDNA sequencing, short-chain fatty acids (SCFAs), and bioinformatics were analyzed with the colonic tissue status. The results showed that the intake of HPIDF affected the proliferation of the key bacteria , , and in the PWY-2941 pathway and AEROBACTINSYN-PWY pathway, and then affected the synthesis of SCFAs, providing a positive role for colon health. However, the intake of HPIDF was unable to repair colonic injury caused by DSS-induced acute ulcerative colitis mainly owing to the abundance of in the colon. This study demonstrates that the recommended intake content of HPIDF can ameliorate colonic environment disturbance caused by acute ulcerative colitis, but not enough to relieve it.