Enhanced osteogenic effect in reduced BMP-2 doses with siNoggin transfected pre-osteoblasts in 3D silk scaffolds

[Display omitted] Bone morphogenetic proteins (BMPs), especially BMP-2, are being increasingly used in bone tissue engineering due to its osteo-inductive effects. Although recombinant human BMP-2 (rhBMP-2) was approved by Food and Drug Administration (FDA) to use for bone repair, its high doses caus...

Full description

Saved in:
Bibliographic Details
Published in:International journal of pharmaceutics 2022-01, Vol.612, p.121352-121352, Article 121352
Main Authors: Fuerkaiti, Sümeyra Nur, Çakmak, Anıl Sera, Karaaslan, Cagatay, Gümüşderelioğlu, Menemşe
Format: Article
Language:English
Subjects:
BMP-2
Bone Morphogenetic Protein 2 - genetics
Bone tissue engineering
Cell Differentiation
Humans
Noggin
Osteoblasts
Osteogenesis
Silk
Silk scaffold
siRNA
Tissue Engineering
Tissue Scaffolds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] Bone morphogenetic proteins (BMPs), especially BMP-2, are being increasingly used in bone tissue engineering due to its osteo-inductive effects. Although recombinant human BMP-2 (rhBMP-2) was approved by Food and Drug Administration (FDA) to use for bone repair, its high doses cause undesired side effects. In order to reduce the BMP-2 dose for enhanced osteogenic differentiation, in this study we decided to suppress the synthesis of Noggin protein, the primary antagonist of BMP-2, on the MC3T3-E1 cells using Noggin targeted small interfering RNA (siRNA). Unlike other studies, Noggin siRNA (siNoggin) transfected cells were seeded on silk scaffolds, and osteogenic differentiation was investigated for a long-term period (21 days) with MTT, qPCR, SEM/EDS, and histological analysis. Besides, siNoggin transfected MC3T3-E1 cells were evaluated as a new cell source for tissue engineering studies. It was determined that Nog gene expression was suppressed in the siNoggin group and Ocn gene expression increased 5-fold compared to the control group (*p 
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2021.121352