Cd immobilization mechanisms in a Pseudomonas strain and its application in soil Cd remediation

• Published in: Journal of hazardous materials 2022-03, Vol.425, p.127919-127919, Article 127919
• Main Authors: Wu, Shijuan, Zhou, Zijie, Zhu, Lin, Zhong, Limin, Dong, Yixuan, Wang, Gejiao, Shi, Kaixiang
• Format: Article
• Language: English
• Subjects: Adsorption; Cadmium - analysis; Cadmium binding protein; Cadmium immobilization; Pot experiment; Pseudomonas; Silicate; Soil; Soil Pollutants - analysis
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2021.127919

In this study, we isolated a highly cadmium (Cd)-resistant bacterium, Pseudomonas sp. B7, which immobilized 100% Cd(II) from medium. Culturing strain B7 with Cd(II) led to the change of functional groups, mediating extracellular Cd(II) adsorption. Proteomics showed that a carbonic anhydrase, CadW, was upregulated with Cd(II). CadW expression in Escherichia coli conferred resistance to Cd(II) and increased intracellular Cd(II) accumulation. Fluorescence assays demonstrated that CadW binds Cd(II) and the His123 residue affected Cd(II) binding activity, indicating that CadW participates in intracellular Cd(II) sequestration. Chinese cabbage pot experiments were performed using strain B7 and silicate [Si(IV)]. Compared with the control, Cd content in aboveground parts significantly decreased by 21.3%, 29.4% and 32.9%, and nonbioavailable Cd in soil significantly increased by 129.4%, 45.0% and 148.7% in B7, Si(IV) and B7 +Si(IV) treatments, respectively. The application of Si(IV) alone reduced chlorophyll content by 20.8% and arylsulfatase activity in soil by 33.9%, and increased malonaldehyde activity by 15.0%. The application of strain B7 alleviated the negative effect of Si(IV) on plant and soil enzymes. Overall, application of Si(IV) is most conducive to the decreased Cd accumulation in plant, and strain B7 is beneficial to maintaining soil and plant health. [Display omitted] •Pseudomonas sp. B7 efficiently removed 100% Cd from medium after filtration.•A Cd binding protein, CadW, mediates Cd resistance and intracellular sequestration.•Pseudomonas microbial reagent and silicate decreased Cd content in Chinese cabbage.•Pseudomonas microbial reagent improved Cd immobilization in soil.•Strain B7 alleviated the negative effect of silicate or Cd on plant and soil health.