Solid-state 1H NMR-based metabolomics assessment of tributylin effects in zebrafish bone

• Published in: Life sciences (1973) 2022-01, Vol.289, p.120233-120233, Article 120233
• Main Authors: Ganesan, Raja, Sekaran, Saravanan, Vimalraj, Selvaraj
• Format: Article
• Language: English
• Subjects: Agribusiness; Antifouling substances; Biocides; Biomedical materials; Bone; Catalysis; Danio rerio; Glutamine; Glutathione; Glycolysis; Heat stabilizers; In vivo methods and tests; Mesenchyme; Metabolic profiling; Metabolism; Metabolites; Metabolomics; NMR; NMR spectroscopy; Nuclear magnetic resonance; Osteoclasts; Solid state; Spectroscopy; Stem cells; Tributyltin; Tributyltin compounds; Tricarboxylic acid cycle; Trimethylamine; Zebrafish; Zebrafish (Danio rerio)
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2021.120233

Tributyltin (TBT), an endocrine disruptor is used globally in agribusiness and industries as biocides, heat stabilizers, and in chemical catalysis. It is known for its deleterious effects on bone by negatively impacting the functions of osteoblasts, osteoclasts and mesenchymal stem cells. However, the impact of TBT on the metabolomics profile in bone is not yet studied. Here, we demonstrate alterations in chemical metabolomics profiles measured by solid state 1H nuclear magnetic resonance (1H NMR) spectroscopy in zebrafish bone following tributyltin (TBT) treatment. TBT of 0, 100, 200, 300, 400 and 500 μg/L were exposed to zebrafish. From this, zebrafish bone has subjected for further metabolomics profiling. Samples were measured via one-dimensional (1D) solvent -suppressed and T2- filtered methods with in vivo zebrafish metabolites. A dose dependent alteration in the metabolomics profile was observed and results indicated a disturbed aminoacid metabolism, TCA cycle, and glycolysis. We found a significant alteration in the levels of glutamate, glutamine, glutathione, trimethylamine N-oxide (TMAO), and other metabolites. This investigation hints us the deleterious effects of TBT on zebrafish bone enabling a comprehensive understanding of metabolomics profile and is expected to play a crucial role in understanding the deleterious effects of various endocrine disruptor on bone. [Display omitted] •The metabolic changes of tributyltin (TBT) on zebrafish were studied using high-throughput screening.•TBT influenced metabolic alterations in bone metabolisms.•TBT has inhibited metabolic activity of energy, phospholipids, and neuroprotective pathways.•TBT induced changes and quantitates the variability of metabolites and metabolic pathways.