A Simple Table-Top Technique for Multi-Signal Pseudo-Extracellular Recording

Validation of neural probe performance often includes implantation in live animals, to assess ability to detect and distinguish signals generated by individual neurons. While this method is informative, an effective in vitro alternative would streamline device development and improve ethical conside...

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Bibliographic Details
Main Authors: Niemiec, Martin. J., Han, Martin
Format: Conference Proceeding
Language:English
Subjects:
Animals
Microelectrodes
Neural engineering
Neurons
Performance evaluation
Physiology
Probes
Online Access:Request full text
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Summary:Validation of neural probe performance often includes implantation in live animals, to assess ability to detect and distinguish signals generated by individual neurons. While this method is informative, an effective in vitro alternative would streamline device development and improve ethical considerations by reducing the use of animals in the validation of neural recording devices. Here, we describe a simple system using ball electrodes to apply multiple neural waveforms to phosphate buffered saline, which are simultaneously recorded by a microelectrode probe. Using this technique, our neural probe was able to detect and distinguish spikes from multiple units of roughly physiological amplitudes (~100 microvolts peak to peak), indicating promise as an in vitro alternative to animal testing for initial validation of neural recording devices.
ISSN:1948-3546
1948-3554
DOI:10.1109/NER49283.2021.9441208