A dual responsive robust human serum albumin‐based nanocarrier for doxorubicin

Targeted drug therapy against cancer has been introduced as a smart strategy to combat the unwanted side effects due to systemic administration of chemotherapeutics. A human serum albumin (HSA)‐based nanocarrier was fabricated with the aim to target reductive media and acidic pH of the tumor tissues...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2022-12, Vol.69 (6), p.2496-2506
Main Authors: Toosi Moghadam, Fatemeh, Mamashli, Fatemeh, Khoobi, Mehdi, Ghasemi, Atiyeh, Pirhaghi, Mitra, Delavari, Behdad, Mahmoudi Aznaveh, Hooman, Nikkhah, Maryam, Saboury, Ali Akbar
Format: Article
Language:English
Subjects:
active targeting
Albumin
Chemotherapy
Disulfide bonds
Doxorubicin
Drug Carriers - chemistry
Drug Delivery Systems
Drug therapy
Ethanol
Glutathione
Human serum albumin
Humans
Hydrogen-Ion Concentration
Infrared analysis
Infrared spectroscopy
Lipoic acid
Nanoparticles
Nanoparticles - chemistry
Nanotechnology
Neoplasms
pH effects
Plasma proteins
Polyethylene glycol
Polyethylene Glycols - chemistry
Proteins
reduction responsive
Serum albumin
Serum Albumin, Human
Shelf life
Side effects
Storage stability
Tumors
α‐lipoic acid
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Summary:Targeted drug therapy against cancer has been introduced as a smart strategy to combat the unwanted side effects due to systemic administration of chemotherapeutics. A human serum albumin (HSA)‐based nanocarrier was fabricated with the aim to target reductive media and acidic pH of the tumor tissues. α‐Lipoic acid (LA) was applied to increase the number of disulfide bonds in the nanocarrier to target higher glutathione concentrations present in tumor tissues and polyethylene glycol was used to target the acidic pH of tumors. UV illumination, ethanol desolvation, oxygen bubbling, and a mixture of redox buffers were employed to prepare doxorubicin‐loaded HSA–LA nanoparticles. The nanocarrier was supposed to release the loaded doxorubicin in reductive and acidic pH media. Fourier‐transform infrared spectroscopy and energy dispersive X‐ray analysis indicated successful attachment of LA to HSA. The prepared nanoplatform presented improved doxorubicin loading efficiency and content and successfully released the loaded doxorubicin in the expected conditions. Protein corona study indicated that positively charged plasma proteins with molecular weights of nearly 80 kDa are absorbed to the surface of the nanoparticles. Furthermore, it showed desirable UV and storage stability, which implied its robustness and improved shelf life if applied in nanomedicine.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2299