Bcl-2 Interacts with Beclin 1 and Regulates Autophagy in 7, 12-Dimethylbenz[a]anthracene-Induced Hamster Buccal-Pouch Squamous-Cell Tumorigenesis

Objective Autophagy is a programmed cell death procedure, which has essential functions in tumorigenesis. However, its temporal expression and function under different status are yet to be determined. This study aims to investigate the temporal expression of autophagy and its possible function in 7,...

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Bibliographic Details
Published in:Current medical science 2021-12, Vol.41 (6), p.1198-1204
Main Authors: Liu, Qian, Liu, Yang, Li, Shu-e, Geng, Jin-huan
Format: Article
Language:English
Subjects:
9,10-Dimethyl-1,2-benzanthracene - adverse effects
Animals
Apoptosis
Autophagy
Beclin-1 - metabolism
Carcinogenesis
Carcinogens
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Cheek - pathology
Cricetinae
Epithelial Cells - metabolism
Immunohistochemistry
Medicine
Medicine & Public Health
Mouth Neoplasms - genetics
Mouth Neoplasms - pathology
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
Time Factors
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Summary:Objective Autophagy is a programmed cell death procedure, which has essential functions in tumorigenesis. However, its temporal expression and function under different status are yet to be determined. This study aims to investigate the temporal expression of autophagy and its possible function in 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal-pouch cancer model (HBPCM). Methods A total of 50 hamster buccal-pouch tumorigenesis models were established by painting DMBA for 4, 8, 10 and 13 weeks. The expression and subcellular localization of LC3, Beclin 1 and Bcl-2 in buccal lesions were evaluated by immunohistochemical staining and Western blotting. DNA damage was observed by immunohistochemical staining of 8-oHdG. The relationship between Beclin 1 and Bcl-2 was analyzed by immunofluorescence colocalization. Results The expression levels of LC3 and Beclin 1 associated with autophagy in the experimental buccal pouch of HBPCM were significantly upregulated after 4 weeks ( P
ISSN:2096-5230
2523-899X
DOI:10.1007/s11596-021-2472-5