Acute toxicity of methomyl commercial formulation induces morphological and behavioral changes in larval zebrafish (Danio rerio)

The use of pesticides has continue grown over recent years, leading to several environmental and health concerns, such as the contamination of surface and groundwater resources and associated biota, potentially affecting populations that are not primary targets of these complex chemical mixtures. In...

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Bibliographic Details
Published in:Neurotoxicology and teratology 2022-01, Vol.89, p.107058-107058, Article 107058
Main Authors: Jablonski, Camilo Alexandre, Pereira, Talita Carneiro Brandão, Teodoro, Lilian De Souza, Altenhofen, Stefani, Rübensam, Gabriel, Bonan, Carla Denise, Bogo, Maurício Reis
Format: Article
Language:English
Subjects:
Acetylcholinesterase - pharmacology
Animals
Carbamate pesticides
Ecotoxicology
Embryo, Nonmammalian
FET
Larva
Methomyl
Water Pollutants, Chemical - toxicity
Zebrafish - physiology
Zebrafish behavior
Zebrafish morphology
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Summary:The use of pesticides has continue grown over recent years, leading to several environmental and health concerns, such as the contamination of surface and groundwater resources and associated biota, potentially affecting populations that are not primary targets of these complex chemical mixtures. In this work, we investigate lethal and sublethal effects of acute exposure of methomyl commercial formulation in zebrafish embryo and larvae. Methomyl is a broad-spectrum carbamate insecticide and acaricide that acts primarily in acetylcholinesterase inhibition (AChE). Methomyl formulation 96 h-LC50 was determined through the Fish Embryo Acute Toxicity Test (FET) and resulted in 1.2 g/L ± 0.04. Sublethal 6-day exposure was performed in six methomyl formulation concentrations (0.5; 1.0; 2.2; 4.8; 10.6; 23.3 mg/L) to evaluate developmental, physiological, morphological, behavioral, biochemical, and molecular endpoints of zebrafish early-development. Methomyl affected embryo hatching and larva morphology and behavior, especially in higher concentrations; resulting in smaller body and eyes size, failure in swimming bladder inflation, hypolocomotor activity, and concentration-dependent reduction of AChE activity; demonstrating methomyl strong acute toxicity and neurotoxic effect. [Display omitted] •Methomyl formulation LC50 for embryos was higher than reported for adult zebrafish.•Morphological changes identified in 5 dpf larvae exposed to methomyl formulation.•Methomyl formulation induced hypolocomotor behavior in 6 dpf zebrafish.
ISSN:0892-0362
1872-9738
DOI:10.1016/j.ntt.2021.107058