Ultrathin Ruthenium Nanosheets with Crystallinity-Modulated Peroxidase-like Activity for Protein Discrimination

Noble-metal-based nanozymes have attracted great interest as enzyme mimics because of their unique properties. To modulate the performance and meet the requirements of practical biosensing applications, phase engineering is promising for the design of novel noble-metal-based nanomaterials. Herein, a...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-01, Vol.94 (2), p.1022-1028
Main Authors: Tang, Yinjun, Wu, Yu, Xu, Weiqing, Jiao, Lei, Chen, Yifeng, Sha, Meng, Ye, Hua-Rong, Gu, Wenling, Zhu, Chengzhou
Format: Article
Language:English
Subjects:
Analytical chemistry
Arrays
Biosensors
Catalysis
Chemistry
Colorimetry
Crystal structure
Crystallinity
Nanomaterials
Nanosheets
Nanostructures - chemistry
Nanotechnology
Noble metals
Peroxidase
Peroxidases
Proteins
Ruthenium
Substrate inhibition
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Noble-metal-based nanozymes have attracted great interest as enzyme mimics because of their unique properties. To modulate the performance and meet the requirements of practical biosensing applications, phase engineering is promising for the design of novel noble-metal-based nanomaterials. Herein, a simple salt-assist strategy was employed for the synthesis of Ru nanosheets (NSs) with the controlled crystalline degree. The crystalline degree plays a significant role in tuning peroxidase-like activity by optimizing the affinity toward the catalytic substrate. Furthermore, the inhibition effect of mercapto molecules on the peroxidase-like activity of Ru NSs was investigated. As a proof-of-concept, the Ru NSs-based colorimetric sensing arrays were developed to distinguish mercapto molecules, and five model molecules were well classified according to the different inhibition effects. Given the complexity of practical conditions, the sensing array was further applied to discriminate proteins possessing rich mercapto groups. This work not only provides an effective strategy for the design of highly active nanozymes but also achieves promising sensing arrays for practical needs.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c03987