Effect of Alpha-Fetoprotein on Differentiation of Myeloid Supressor Cells

The effect of recombinant alpha-fetoprotein (AFP) on human myeloid suppressor cell (MDSC) differentiation was studied in vitro in the presence of cytokines IL-6 (10 ng/mL) and GM-CSF (10 ng/mL). It was found that AFP at concentrations of 50 and 100 IU/mL increased the number of MDSC (CD33 + HLA-DR –...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2021-11, Vol.501 (1), p.434-437
Main Authors: Zamorina, S. A., Shardina, K. Yu, Timganova, V. P., Bochkova, M. S., Uzhviyuk, S. V., Raev, M. B., Chereshnev, V. A.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
CD11b antigen
CD14 antigen
Cell culture
Cell differentiation
Granulocyte-macrophage colony-stimulating factor
Interleukin 6
Life Sciences
Molecular Biology
Monocytes
α-Fetoprotein
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Summary:The effect of recombinant alpha-fetoprotein (AFP) on human myeloid suppressor cell (MDSC) differentiation was studied in vitro in the presence of cytokines IL-6 (10 ng/mL) and GM-CSF (10 ng/mL). It was found that AFP at concentrations of 50 and 100 IU/mL increased the number of MDSC (CD33 + HLA-DR –/low CD11b + ) in culture. Analysis of MDSC subpopulations showed that the increase was due to monocytic M-MDSC (HLA-DR –/low CD33 + CD11b + CD14 + CD66b – ). There was no modulating effect of AFP on granulocytic PMN-MDSC (HLA-DR –/low CD33 + CD11b + CD14 – CD66b + ). The effects of recombinant AFP on MDSC differentiation were thus demonstrated for the first time.
ISSN:1607-6729
1608-3091
1608-3091
DOI:10.1134/S1607672921060077