A UPLC-MS/MS method for the determination of oxidative stress biomarkers in amniotic fluid

Oxidative stress in the fetal period is associated with preterm birth as well as short and long-term adverse clinical outcomes. Here, an Ultra-Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived...

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Bibliographic Details
Published in:Free radical biology & medicine 2022-02, Vol.179, p.164-169
Main Authors: Cascant-Vilaplana, Mari Merce, Albiach-Delgado, Abel, Camprubí-Camprubí, Marta, Pérez-Cruz, Miriam, Gómez, Olga, Arráez, Miguel, López-Nogueroles, Marina, Kuligowski, Julia, Vento, Máximo
Format: Article
Language:English
Subjects:
Amniotic Fluid
Biomarkers
Chromatography, High Pressure Liquid
Chromatography, Liquid
DNA oxidation
Female
Humans
Infant, Newborn
Method validation
Oxidative Stress
Pregnancy
Premature Birth
Protein oxidation
Reactive oxygen species (ROS)
Tandem Mass Spectrometry
Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS)
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Summary:Oxidative stress in the fetal period is associated with preterm birth as well as short and long-term adverse clinical outcomes. Here, an Ultra-Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived damage to proteins and DNA in amniotic fluid (AF) samples is presented. Appropriate accuracy and precision levels, as well as sensitivity with limits of detection in the low nanomolar (
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2021.12.310