A UPLC-MS/MS method for the determination of oxidative stress biomarkers in amniotic fluid

Oxidative stress in the fetal period is associated with preterm birth as well as short and long-term adverse clinical outcomes. Here, an Ultra-Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived...

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Published in:Free radical biology & medicine 2022-02, Vol.179, p.164-169
Main Authors: Cascant-Vilaplana, Mari Merce, Albiach-Delgado, Abel, Camprubí-Camprubí, Marta, Pérez-Cruz, Miriam, Gómez, Olga, Arráez, Miguel, López-Nogueroles, Marina, Kuligowski, Julia, Vento, Máximo
Format: Article
Language:English
Subjects:
Amniotic Fluid
Biomarkers
Chromatography, High Pressure Liquid
Chromatography, Liquid
DNA oxidation
Female
Humans
Infant, Newborn
Method validation
Oxidative Stress
Pregnancy
Premature Birth
Protein oxidation
Reactive oxygen species (ROS)
Tandem Mass Spectrometry
Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS)
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container_title Free radical biology & medicine
container_volume 179
creator Cascant-Vilaplana, Mari Merce
Albiach-Delgado, Abel
Camprubí-Camprubí, Marta
Pérez-Cruz, Miriam
Gómez, Olga
Arráez, Miguel
López-Nogueroles, Marina
Kuligowski, Julia
Vento, Máximo
description Oxidative stress in the fetal period is associated with preterm birth as well as short and long-term adverse clinical outcomes. Here, an Ultra-Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived damage to proteins and DNA in amniotic fluid (AF) samples is presented. Appropriate accuracy and precision levels, as well as sensitivity with limits of detection in the low nanomolar (
doi_str_mv 10.1016/j.freeradbiomed.2021.12.310
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Here, an Ultra-Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of biomarkers of oxidative stress-derived damage to proteins and DNA in amniotic fluid (AF) samples is presented. Appropriate accuracy and precision levels, as well as sensitivity with limits of detection in the low nanomolar (&lt;2 nM) range were achieved. The analytical method was applied to a set of AF samples and reference ranges of the biomarker panel are presented. Median concentrations of biomarkers of protein oxidation (ortho-, 3-chloro-, and 3-nitrotyrosine) and their precursors (para-tyrosine and phenylalanine) ranged between 0.6 and 3 nM and 23 and 30 μM, respectively, while levels of a biomarker of DNA-oxidation (8-hydroxydeoxyguanosine, 8OHdG) and its precursor (2′-deoxyguanosine) were found to be 0.18 and 3 nM, respectively. 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Detection frequencies of all metabolites were 100% with exception of 3-chlorotyrosine (3Cl-Tyr) and 8OHdG, that were only detected in 8% of samples. The developed method may be applied in research studies focusing on oxidative stress-related complications during pregnancy. [Display omitted] •Simultaneous measurement of oxidative damage to proteins and DNA in amniotic fluid.•Validation of a UPLC-MS/MS method for oxidative stress biomarker quantification.•Small sample volumes (i.e., 250 μL) and high sensitivity (LODs &lt;2 nM).•Reference ranges for oxidative biomarker concentrations in amniotic fluid.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34971756</pmid><doi>10.1016/j.freeradbiomed.2021.12.310</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-6979-2235</orcidid></addata></record>
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subjects Amniotic Fluid
Biomarkers
Chromatography, High Pressure Liquid
Chromatography, Liquid
DNA oxidation
Female
Humans
Infant, Newborn
Method validation
Oxidative Stress
Pregnancy
Premature Birth
Protein oxidation
Reactive oxygen species (ROS)
Tandem Mass Spectrometry
Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS)
title A UPLC-MS/MS method for the determination of oxidative stress biomarkers in amniotic fluid
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