Environmental Enrichment Differentially Activates Neural Circuits in FVB/N Mice, Inducing Social Interaction in Females but Agonistic Behavior in Males

• Published in: Neurochemical research 2022-03, Vol.47 (3), p.781-794
• Main Authors: Cabrera-Muñoz, Edith Araceli, Olvera-Hernández, Sandra, Vega-Rivera, Nelly Maritza, Meneses-San Juan, David, Reyes-Haro, Daniel, Ortiz-López, Leonardo, Ramírez Rodríguez, Gerardo Bernabé
• Format: Article
• Language: English
• Subjects: Aggression - physiology; Aggressive behavior; Agonistic behavior; Agonistic Behavior - physiology; Amygdala; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Circuits; Cortex (frontal); Cortex (insular); Enrichment; Environmental effects; Female; Females; Hippocampus; Housing; Limbic system; Male; Males; Mice; Mice, Inbred Strains; Neural networks; Neurochemistry; Neurogenesis; Neurology; Neuroplasticity; Neurosciences; Original Paper; Rodents; Sex differences; Sexual dimorphism; Social Behavior; Social factors; Social Interaction
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-021-03487-8

Environmental enrichment induces behavioral and structural modifications in rodents and influences the capability of mice to cope with stress. However, little is understood about hippocampal neurogenesis and the appearance of social/agonistic (aggressive) behavior upon activation of different neuronal circuits in FVB/N mice. Thus, in this study we hypothesized that environmental enrichment differentially regulates neurogenesis, neural circuit activation and social/agonistic behavior in male and female FVB/N mice. We explored the (1) neurogenic process as an indicative of neuroplasticity, (2) neuronal activation in the limbic system, and (3) social behavior using the resident-intruder test. On postnatal day 23 (PD23), mice were assigned to one of two groups: Standard Housing or Environmental Enrichment. At PD53, rodents underwent the resident-intruder test to evaluate social behaviors. Results revealed that environmental enrichment increased neurogenesis and social interaction in females. In males, environmental enrichment increased neurogenesis and agonistic behavior. Enriched male mice expressed higher levels of agonistic-related behavior than female mice housed under the same conditions. Neural circuit analysis showed lower activation in the amygdala of enriched males and higher activation in enriched females than their respective controls. Enriched females also showed higher activation in the frontal cortex without differences in male groups. Moreover, the insular cortex was less activated in females than in males. Thus, our results indicate that environmental enrichment has different effects on neuroplasticity and social/agonistic behavior in FVB/N mice, suggesting the relevance of sexual dimorphism in response to environmental stimuli.